Identification of Dermatophytosis Agents in Mashhad, Iran, by Using Polymerase
Chain Reaction Sequencing (PCR Sequencing) Method

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Nejati Hoseini Raheleh; ZARRINFAR HOSSEIN; Parian Mahmoud; Parham Saeid; FATA ABDOLMAJID; Matehkolaei Ali Rezaei; NAJAFZADEH MOHAMMAD JAVAD

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S) Year:2019 | Volume:37 | Issue:520 Page(s): 256-262

Download Full-Text

Persian Verion

View:

443

Download:

Cites:

Information Journal Paper

Title

Identification of Dermatophytosis Agents in Mashhad, Iran, by Using Polymerase Chain Reaction Sequencing (PCR Sequencing) Method

Author(s)

Keywords

DermatophytosisQ3

DNA sequencingQ2

IranQ2

Abstract

Background: Dermatophytes are a group of fungi that attack keratinous tissues of the skin, hair, and nail in humans and animals, and cause infections called Dermatophytosis (tinea). Since identification of pathogenic fungi at the species level is essential for the detection of the source, control and prevention, and identifying epidemiology of infection, it is necessary to use specific and sensitive diagnostic methods to identify the causes of Dermatophytosis. Methods: The clinical samples (skin, nail, and hair) of patients with Dermatophytosis in Mashhad City, Iran, were cultured in Mycosyl Agar culture media, and the DNA of obtained dermatophyte colonies were extracted by specific kit. The internal transcribed spacer (ITS) gene was amplified and sequenced by ITS1, ITS4 primers. Finally, the sequencing results were analyzed using SeqMan software, and were compared with the data of the global genebank. Findings: In this study, 80 dermatophyte isolates were sequenced, which included 9 dermatophyte species as 23 (28. 8%) Trichophyton (T. ) interdigital, 18 (22. 5%) T. tunsorans, 10 (12. 5%) Epidermophyton fluccosum, 10 (12. 5%) of T. mentagrophytes, 8 (10%) Microsporum canis, 4 (5%) T. rubrum, 4 (5%) T. benhamiae, 2 (2. 5%) Nannizzia (N. ) fulvum, 1 (1. 2%) N. persicolor. Conclusion: According to report the rare species of dermatophytes in this study, the use of molecular methods such as sequencing of the ITS gene can determine the diversity of dermatophytes in a region more precisely than morphological methods.

Cites

Low in vitro activity of sertaconazole against clinical isolates of dermatophyte

References

No record.

Cite

APA: Copy

Nejati Hoseini, Raheleh, ZARRINFAR, HOSSEIN, Parian, Mahmoud, Parham, Saeid, FATA, ABDOLMAJID, Matehkolaei, Ali Rezaei, & NAJAFZADEH, MOHAMMAD JAVAD. (2019). Identification of Dermatophytosis Agents in Mashhad, Iran, by Using Polymerase Chain Reaction Sequencing (PCR Sequencing) Method. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), 37(520 ), 256-262. SID. https://sid.ir/paper/395634/en

Vancouver: Copy

Nejati Hoseini Raheleh, ZARRINFAR HOSSEIN, Parian Mahmoud, Parham Saeid, FATA ABDOLMAJID, Matehkolaei Ali Rezaei, NAJAFZADEH MOHAMMAD JAVAD. Identification of Dermatophytosis Agents in Mashhad, Iran, by Using Polymerase Chain Reaction Sequencing (PCR Sequencing) Method. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)[Internet]. 2019;37(520 ):256-262. Available from: https://sid.ir/paper/395634/en

IEEE: Copy

Raheleh Nejati Hoseini, HOSSEIN ZARRINFAR, Mahmoud Parian, Saeid Parham, ABDOLMAJID FATA, Ali Rezaei Matehkolaei, and MOHAMMAD JAVAD NAJAFZADEH, “Identification of Dermatophytosis Agents in Mashhad, Iran, by Using Polymerase Chain Reaction Sequencing (PCR Sequencing) Method,” JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), vol. 37, no. 520 , pp. 256–262, 2019, [Online]. Available: https://sid.ir/paper/395634/en

Related Journal Papers