Isolation, culture and differentiation of human spermatogonial stem cells into differentiated germ cells, osteoblasts and adipocytes

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Ashouri Sanaz; Feraghat Sahar; FARGHADAN MARYAM; Ashouri Sepideh

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Journal: Razi Journal of Medical Sciences Year:2019 | Volume:26 | Issue:9 Page(s): 1-9

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Title

Isolation, culture and differentiation of human spermatogonial stem cells into differentiated germ cells, osteoblasts and adipocytes

Author(s)

Ashouri Sanaz | Feraghat Sahar | FARGHADAN MARYAM | Ashouri Sepideh | Issue Writer Certificate

Keywords

Spermatogonial stem cellsQ2

ProliferationQ2

DifferentiationQ1

Abstract

Background: Spermatogonial stem cells (SSCs) differentiate into adult germ cells during spermatogenesis and cause male fertility. Maintenance of SSCs and induction of spermiogenesis in vitro may provide a therapeutic strategy to treat male infertility. Moreover, it is possible to use the SSCs for research in the field of biotechnology and regenerative medicine through Differentiation of them into other cell lines. Methods: SSCs were isolated enzymatically from digested testicular tissue and then were cultured. The identity of SSCs were confirmed by anti-OCT4, anti-PLZF through immunocytochemistry. SSCs were cultured in differentiated culture medium. Then, expression level of SCP3, Boule, Crem, Protamine 2 genes and also expression level of SCP3, Protamine2 proteins was determined by Real-time PCR and immunocytochemistry, respectively. In addition, the potency of SSCs Differentiation into osteoblasts and adipocytes was evaluated by Alizarin Red and Oil Red staining, respectively. Results: The results showed that SSCs can differentiate into adult germ cells in in vitro condition. Also, under Differentiation conditions, these cells can be distinguished from other cell lines. Conclusion: The results of this study showed that, given the Differentiation potency of SSCs, these cells can be used for the treatment of male infertility, as well as cell therapy and regenerative medicine.

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APA: Copy

Ashouri, Sanaz, Feraghat, Sahar, FARGHADAN, MARYAM, & Ashouri, Sepideh. (2019). Isolation, culture and differentiation of human spermatogonial stem cells into differentiated germ cells, osteoblasts and adipocytes. RAZI JOURNAL OF MEDICAL SCIENCES (JOURNAL OF IRAN UNIVERSITY OF MEDICAL SCIENCES), 26(9 ), 1-9. SID. https://sid.ir/paper/395675/en

Vancouver: Copy

Ashouri Sanaz, Feraghat Sahar, FARGHADAN MARYAM, Ashouri Sepideh. Isolation, culture and differentiation of human spermatogonial stem cells into differentiated germ cells, osteoblasts and adipocytes. RAZI JOURNAL OF MEDICAL SCIENCES (JOURNAL OF IRAN UNIVERSITY OF MEDICAL SCIENCES)[Internet]. 2019;26(9 ):1-9. Available from: https://sid.ir/paper/395675/en

IEEE: Copy

Sanaz Ashouri, Sahar Feraghat, MARYAM FARGHADAN, and Sepideh Ashouri, “Isolation, culture and differentiation of human spermatogonial stem cells into differentiated germ cells, osteoblasts and adipocytes,” RAZI JOURNAL OF MEDICAL SCIENCES (JOURNAL OF IRAN UNIVERSITY OF MEDICAL SCIENCES), vol. 26, no. 9 , pp. 1–9, 2019, [Online]. Available: https://sid.ir/paper/395675/en

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