Preparation of biological scaffolds derived from bladder sheep and evaluation of Bio compatibility and mechanical properties of the scaffold

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Najafi Zangir r.; ASADI A.; ZAHRI S.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF CELL & TISSUE Year:2019 | Volume:10 | Issue:3 Page(s): 181-192

Download Full-Text

Persian Verion

View:

524

Download:

Cites:

Information Journal Paper

Title

Preparation of biological scaffolds derived from bladder sheep and evaluation of Bio compatibility and mechanical properties of the scaffold

Author(s)

Najafi Zangir r. | ASADI A. | ZAHRI S. | Issue Writer Certificate

Keywords

scaffoldQ2

urinary bladderQ1

mesenchymal stem cellsQ1

biocompatibilityQ2

Abstract

Aim: This study was aimed to preparation of sheep urinary bladder derived from biological scaffold by a combined (physical and chemical) method and evaluation of scaffold biocompatibility. Materials and Methods: urinary bladder decellularization was performed by physical and chemical methods. In the physical method, the bladder fragments were incubated at-4 ° C for 24 h and intervaled the fragments each 6 h by placing for 10 min in 0. 1% sodium azide solution. After 24 h, the samples were placed at-20 and-40 ° C for 2 and 1 h, respectively. The bladder fragments were held in a cryotube and emerged in liquid nitrogen by five two-minute steps, and finally washed by the PBS buffer containing 0. 1% sodium azide. In the chemical decellularization, all of the physically treated bladder fragments were placed in a sodium dodecyl sulphate solution under slow stirring for 24 h. The samples were rinsed with sterile distilled water, sterilized with 75% ethanol and 0. 2% peracetic acid and finally were placed in PBS for 24 h. Results: The light and electron microscopey studies revealed the biocompatibility of seeded stem cells on the sheep bladder bioscaffold, in 3rd, 5th and 7th days. The most biocompatibility was observed in the end of 7th day. Conclusion: Decellularized urinary bladder scaffold revealed biocompatibility which could be considered as a potential nontoxic and biocompatible bioscaffold for application in tissue engineering regenerative medicine.

Cites

No record.

References

No record.

Cite

APA: Copy

Najafi Zangir, r., ASADI, A., & ZAHRI, S.. (2019). Preparation of biological scaffolds derived from bladder sheep and evaluation of Bio compatibility and mechanical properties of the scaffold. JOURNAL OF CELL & TISSUE, 10(3 ), 181-192. SID. https://sid.ir/paper/395965/en

Vancouver: Copy

Najafi Zangir r., ASADI A., ZAHRI S.. Preparation of biological scaffolds derived from bladder sheep and evaluation of Bio compatibility and mechanical properties of the scaffold. JOURNAL OF CELL & TISSUE[Internet]. 2019;10(3 ):181-192. Available from: https://sid.ir/paper/395965/en

IEEE: Copy

r. Najafi Zangir, A. ASADI, and S. ZAHRI, “Preparation of biological scaffolds derived from bladder sheep and evaluation of Bio compatibility and mechanical properties of the scaffold,” JOURNAL OF CELL & TISSUE, vol. 10, no. 3 , pp. 181–192, 2019, [Online]. Available: https://sid.ir/paper/395965/en

Related Journal Papers