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Title

Apoptotic effect of apoptin gene transduction on multiple myeloma cell line

Pages

  324-330

Abstract

 Introduction: Following the first description of Multiple myeloma (MM), as the second most prevalent hematologic malignancy, multiple promising advances have paved the way to increase the long-lasting complete remission for patients. In the era of the novel therapeutic approaches, the cloning of the apoptosis-inducing genes into the genome of malignant cells has attracted tremendous attention. In this study, we aimed to evaluate the anti-cancer effect of Apoptin-expressing vector on Multiple myeloma-derived KMM-1 cell line. Materials and Methods: In this experimental study, we constructed the lenti-viral vector expressing Apoptin for transferring this gene into KMM-1 cells. Transduction efficiency and Apoptin gene expression were further confirmed by flow cytometric and quantitative real-time PCR analysis, respectively. Afterwards, the anti-survival effect of overexpressed Apoptin on KMM-1 cells was also evaluated by applying MTT, and trypan blue assays. Results: The results of flow cytometric analysis demonstrated that the Apoptin-expressing vector was successfully transduced into KMM-1 cells, with the transduction efficiency of more than 90%. In addition, we found that the overexpression of Apoptin, as revealed by 8-fold increase in its mRNA level, exerted a remarkable cytotoxic effect in MM cells. The resulting data declared that Apoptin expression not only significantly reduced cell viability (P < 0, 001) but also potently halted KMM-1 metabolic activity (P < 0. 001). Conclusion: This study highlighted the promising anti-cancer effect of lenti-viral vector expressing Apoptin in Multiple myeloma that may be clinically accessible in the near future.

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    APA: Copy

    Anjam Najmedini, Ali, VAHABPOUR ROUDSARI, ROUHOLLAH, POURBAGHERI SIGAROODI, ATIEH, Sorourian, Soofia, & BASHASH, DAVOOD. (2019). Apoptotic effect of apoptin gene transduction on multiple myeloma cell line. KOOMESH, 21(2 (74) ), 324-330. SID. https://sid.ir/paper/396617/en

    Vancouver: Copy

    Anjam Najmedini Ali, VAHABPOUR ROUDSARI ROUHOLLAH, POURBAGHERI SIGAROODI ATIEH, Sorourian Soofia, BASHASH DAVOOD. Apoptotic effect of apoptin gene transduction on multiple myeloma cell line. KOOMESH[Internet]. 2019;21(2 (74) ):324-330. Available from: https://sid.ir/paper/396617/en

    IEEE: Copy

    Ali Anjam Najmedini, ROUHOLLAH VAHABPOUR ROUDSARI, ATIEH POURBAGHERI SIGAROODI, Soofia Sorourian, and DAVOOD BASHASH, “Apoptotic effect of apoptin gene transduction on multiple myeloma cell line,” KOOMESH, vol. 21, no. 2 (74) , pp. 324–330, 2019, [Online]. Available: https://sid.ir/paper/396617/en

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