Promoter Methylation and Expression Status of Cytotoxic T-Lymphocyte-Associated Antigen-4 Gene in Patients with Lupus

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NOSRATZEHI SHAHIN; Nosrat zehi Mahin; Atighi Sahar; Dianat Tahereh; Kord Tamandani Khadije

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Journal: Journal of Epigenetics Year:2020 | Volume:2 | Issue:1 Page(s): 33-39

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Information Journal Paper

Title

Promoter Methylation and Expression Status of Cytotoxic T-Lymphocyte-Associated Antigen-4 Gene in Patients with Lupus

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Keywords

Systemic lupus erythematous

CTLA4 gene

Polymorphism

Promoter Methylation

Gene Expression

Abstract

Objectives: Systemic lupus erythematous (SLE) is an autoimmune disease with both genetic susceptibility and epigenetic modifications. Autoantibodies directly contribute to the destruction of some organs such as kidneys, joints, skin, lungs, central nervous system, and blood-forming (hematopoietic) system. The CTLA4 plays an important role in inhibition of the activity of T cells and preventing autoimmune disorders, for example; the lupus. We analyzed the Promoter Methylation, Polymorphism, and expression status in CTLA4 gene in patients with lupus. Methods: Genomic DNA was isolated from 50 individuals’ blood samples with SLE and 50 control participants. CTLA4 gene Polymorphisms analysis in polymorphic site,-318(CT) and +49(AG), was done by Tetra-ARMS-PCR. Methylation-specific polymerase chain reaction (MS-PCR) was used to estimate promoter hyper methylation of the CTLA4 gene. The present paper also analyzed CTLA4 mRNA levels in 30 blood samples from the intended participants, and healthy control by real-time PCR. Results: Changes in Promoter Methylation of CTLA4 gene were remarkably different in patients with lupus than healthy controls (OR= 0. 48; 95% CI= 0. 1959, 1. 202; P-value= 0. 005). However, Gene Expression level of CTLA4 was not statistically different in patients than the healthy control group. Conclusions: This epigenetic study gives us an overview of the role of CTLA4 Promoter Methylation in pathogenesis of SLE, which cause preventing its expression. As we know CTLA4 has the role in immune regulation and downregulates immune responses. In the future a comprehensive understanding of the epigenetic mechanisms contributing to SLE will likely enable development of new therapeutic agents and strategies that target the dysregulated genes or correct the aberrant epigenetic modifications (Epigenetic therapies for SLE).

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APA: Copy

NOSRATZEHI, SHAHIN, Nosrat zehi, Mahin, Atighi, Sahar, Dianat, Tahereh, & Kord Tamandani, Khadije. (2020). Promoter Methylation and Expression Status of Cytotoxic T-Lymphocyte-Associated Antigen-4 Gene in Patients with Lupus. JOURNAL OF EPIGENETICS, 2(1 ), 33-39. SID. https://sid.ir/paper/397352/en

Vancouver: Copy

NOSRATZEHI SHAHIN, Nosrat zehi Mahin, Atighi Sahar, Dianat Tahereh, Kord Tamandani Khadije. Promoter Methylation and Expression Status of Cytotoxic T-Lymphocyte-Associated Antigen-4 Gene in Patients with Lupus. JOURNAL OF EPIGENETICS[Internet]. 2020;2(1 ):33-39. Available from: https://sid.ir/paper/397352/en

IEEE: Copy

SHAHIN NOSRATZEHI, Mahin Nosrat zehi, Sahar Atighi, Tahereh Dianat, and Khadije Kord Tamandani, “Promoter Methylation and Expression Status of Cytotoxic T-Lymphocyte-Associated Antigen-4 Gene in Patients with Lupus,” JOURNAL OF EPIGENETICS, vol. 2, no. 1 , pp. 33–39, 2020, [Online]. Available: https://sid.ir/paper/397352/en

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