Effect of Leech (Hirudo medicinalis) Saliva on Staphylococcus aureus and Escherichia coli, in vitro

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RASSOULI MARYAM; ESTAJI HAMID; Beykha Mehran; Madah Sepideh; Raeesian Behnaz; Moshtaghi Alireza; Roozbeh Mohammad

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Journal: Iranian Journal of Infectious Diseases and Tropical Medicine Year:2020 | Volume:24 | Issue:87 Page(s): 51-55

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Title

Effect of Leech (Hirudo medicinalis) Saliva on Staphylococcus aureus and Escherichia coli, in vitro

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Keywords

Leech salivaQ1

Leech therapyQ1

Staphylococcus aureusQ2

Escherichia coliQ1

Abstract

Background and objective: In order to increase in microbial resistance, finding biological antimicrobial materials is valuable. Therefore, in this research, antimicrobial effect of Leech saliva on the growth of two bacteria, Staphylococcus aureus (Gram positive) and Escherichia coli (Gram negative) was studied. Materials and methods: The Leech saliva was extracted in vitro and cultured in different dilutions with BHI broth and 1×10 8 S. aureus and E. coli bacteria in sterile microplate. The results were observed and interpreted after 24 and 48 hour incubation at 490 nm wave length. All experiment procedures were repeated 2 weeks later. Results: After 24 hour, in S. aureus culture significant difference was observed among all wells with positive control, in E. coli was just among 1-7 wells (P<0. 05). After 48 hour, again significant difference was observed among all wells of S. aureus with positive control but in E. coli was just in well number 1 (The highest concentration of Leech saliva). Conclusion: According to the results, Leech saliva has antimicrobial effect and more studies on animal models are necessary.

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APA: Copy

RASSOULI, MARYAM, ESTAJI, HAMID, Beykha, Mehran, Madah, Sepideh, Raeesian, Behnaz, Moshtaghi, Alireza, & Roozbeh, Mohammad. (2020). Effect of Leech (Hirudo medicinalis) Saliva on Staphylococcus aureus and Escherichia coli, in vitro. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, 24(87 ), 51-55. SID. https://sid.ir/paper/398030/en

Vancouver: Copy

RASSOULI MARYAM, ESTAJI HAMID, Beykha Mehran, Madah Sepideh, Raeesian Behnaz, Moshtaghi Alireza, Roozbeh Mohammad. Effect of Leech (Hirudo medicinalis) Saliva on Staphylococcus aureus and Escherichia coli, in vitro. IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE[Internet]. 2020;24(87 ):51-55. Available from: https://sid.ir/paper/398030/en

IEEE: Copy

MARYAM RASSOULI, HAMID ESTAJI, Mehran Beykha, Sepideh Madah, Behnaz Raeesian, Alireza Moshtaghi, and Mohammad Roozbeh, “Effect of Leech (Hirudo medicinalis) Saliva on Staphylococcus aureus and Escherichia coli, in vitro,” IRANIAN JOURNAL OF INFECTIOUS DISEASES AND TROPICAL MEDICINE, vol. 24, no. 87 , pp. 51–55, 2020, [Online]. Available: https://sid.ir/paper/398030/en

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