Preparation and Characterization of Smart Poly (N-Isopropylacrylamide) Nanoparticles Containing Vascular Endothelial Growth Factor for Induction of Angiogenesis in Human Bone Marrow-derived Mesenchymal Stem Cells

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ADIBFAR A.; AMOABEDINY GH.; BAGHABAN ESLAMINEJAD M.R.; BAGHERI F.; ZANDIEH DOULABI B.; MOHAMADI J.

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Journal: Pathobiology Research Year:2018 | Volume:21 | Issue:2 Page(s): 65-72

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Title

Preparation and Characterization of Smart Poly (N-Isopropylacrylamide) Nanoparticles Containing Vascular Endothelial Growth Factor for Induction of Angiogenesis in Human Bone Marrow-derived Mesenchymal Stem Cells

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Keywords

Temperature-sensitive PNIPAM NanoparticlesQ1

Nano-precipitationQ1

Growth Factor DeliveryQ1

AngiogenesisQ1

Human Bone Marrow-Derived Mesenchymal Stem CellsQ1

Abstract

Aims Growth factor (GFs) delivery with the certain concentration and release kinetic is one of the main challenges in tissue engineering. The aim of this study was the preparation and characterization of smart poly (N-isopropylacrylamide) nanoparticles containing vascular endothelial growth factor for induction of Angiogenesis in Human Bone Marrow-Derived Mesenchymal Stem Cells. Materials & Methods In this exprimental study, two different formulations of temperaturesensitive Poly (N-isopropylacrylamide) (PNIPAM) nanoparticles (NPs) were synthesized by free radical polymerization technique. Nanoprecipitation and diffusion methods were used to load the vascular endothelial growth factor (VEGF) in PNIPAM NPs. The effects of released VEGF on the differentiation of human bone marrow stem cells (hBMSCs) into endothelial cells in angiogenic, osteogenic, and 50% angiogenic-osteogenic culture medium were investigated, using flow cytometry and light microscope. Statistical analysis was performed, using the GraphPad Prism 6 software. Findings The nanoprecipitation process caused polymer degradation due to using the organic N, N-Dimethylacetamide solvent. The cumulative VEGF released after 72hours for 70%. A total of 10ng/ml VEGF released from PNIPAM nanoparticles, in 2D culture with cell density of 3×104 hBMSCs, after 7 days, leading to the endothelial differentiation, capillary-like tube formation, and expression of 20% vWF as angiogenic marker. Conclusion The PNIPAM NPs have the potential to load and release the angiogenic GFs for induction of Angiogenesis in hBMSCs and in osteogenic medium.

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APA: Copy

ADIBFAR, A., AMOABEDINY, GH., BAGHABAN ESLAMINEJAD, M.R., BAGHERI, F., ZANDIEH DOULABI, B., & MOHAMADI, J.. (2018). Preparation and Characterization of Smart Poly (N-Isopropylacrylamide) Nanoparticles Containing Vascular Endothelial Growth Factor for Induction of Angiogenesis in Human Bone Marrow-derived Mesenchymal Stem Cells. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 21(2 ), 65-72. SID. https://sid.ir/paper/398435/en

Vancouver: Copy

ADIBFAR A., AMOABEDINY GH., BAGHABAN ESLAMINEJAD M.R., BAGHERI F., ZANDIEH DOULABI B., MOHAMADI J.. Preparation and Characterization of Smart Poly (N-Isopropylacrylamide) Nanoparticles Containing Vascular Endothelial Growth Factor for Induction of Angiogenesis in Human Bone Marrow-derived Mesenchymal Stem Cells. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)[Internet]. 2018;21(2 ):65-72. Available from: https://sid.ir/paper/398435/en

IEEE: Copy

A. ADIBFAR, GH. AMOABEDINY, M.R. BAGHABAN ESLAMINEJAD, F. BAGHERI, B. ZANDIEH DOULABI, and J. MOHAMADI, “Preparation and Characterization of Smart Poly (N-Isopropylacrylamide) Nanoparticles Containing Vascular Endothelial Growth Factor for Induction of Angiogenesis in Human Bone Marrow-derived Mesenchymal Stem Cells,” PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), vol. 21, no. 2 , pp. 65–72, 2018, [Online]. Available: https://sid.ir/paper/398435/en

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