Expression and Purification of Recombinant Catalytic Domain of Botulinum Neurotoxin Type E from a Synthetic Gene

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Aghaie S.M.; MOSAVI S.J.; EBRAHIMI F.; SALEHI M.B.; NAZARIAN SH.

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Journal: Pathobiology Research Year:2018 | Volume:21 | Issue:2 Page(s): 79-84

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Information Journal Paper

Title

Expression and Purification of Recombinant Catalytic Domain of Botulinum Neurotoxin Type E from a Synthetic Gene

Author(s)

Keywords

Botulinum toxin Type –Q1

EQ3

Immunoglobulin Light ChainQ2

Catalytic DomainQ1

Recombinant ProteinsQ1

Abstract

Aims Botulinum neurotoxins are the strongest known bacterial toxins that cause muscle paralysis due to inhibition of acetylcholine release. Design of inhibitors is still pursued as a major strategy for intracellular inhibition of poisoning caused by these toxins. Investigation of the potential function of design inhibitors, pure poison or catalytic area is essential. The aims of present study were expression and purification of recombinant Catalytic Domain of botulinum neurotoxin type E (BoNT/E) Type E from a synthetic gene. Materials & Methods In this experimental study, the sequence of the botulinum neurotoxin type E light chain was adopted from GeneBank and codons were optimized according to E. coli BL21 (DE3) codon usage. Other bioinformatics tools were exploited to reach the optimum expression of the gene in the mentioned host. The resultant (gene) was then ordered to synthesize and cloning in pET28a (+) expression vector. The recombinant vector was transferred into E. coli BL21 (DE3) host cells. The expression of the protein was induced by addition of IPTG. The expression conditions were changed to obtain a soluble expression of the protein. Then, the protein was purified by an affinity chromatography, followed by a further purification with amicon filter. SDS-PAGE was used to evaluate expression and purification of the protein and Western blotting was performed to confirm the expressed protein. Findings Codon Adaptation Index of the gene increased to 0. 85. The third predicted structure showed good quality. The thermodynamic analysis of the mRNA structure showed that the predicted structure is stable. The soluble expression was obtained in 18° C and 18h induction by 1 mM IPTG. Protein production with higher more than 90% purity was confirmed. Conclusion Optimization of the protein expression conditions resulted in producing the solution in the culture medium by E. coli BL21 as host.

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APA: Copy

Aghaie, S.M., MOSAVI, S.J., EBRAHIMI, F., SALEHI, M.B., & NAZARIAN, SH.. (2018). Expression and Purification of Recombinant Catalytic Domain of Botulinum Neurotoxin Type E from a Synthetic Gene. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 21(2 ), 79-84. SID. https://sid.ir/paper/398436/en

Vancouver: Copy

Aghaie S.M., MOSAVI S.J., EBRAHIMI F., SALEHI M.B., NAZARIAN SH.. Expression and Purification of Recombinant Catalytic Domain of Botulinum Neurotoxin Type E from a Synthetic Gene. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)[Internet]. 2018;21(2 ):79-84. Available from: https://sid.ir/paper/398436/en

IEEE: Copy

S.M. Aghaie, S.J. MOSAVI, F. EBRAHIMI, M.B. SALEHI, and SH. NAZARIAN, “Expression and Purification of Recombinant Catalytic Domain of Botulinum Neurotoxin Type E from a Synthetic Gene,” PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), vol. 21, no. 2 , pp. 79–84, 2018, [Online]. Available: https://sid.ir/paper/398436/en

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