Comparison of Conventional and Molecular Methods in Identification of Candida Species Isolated from Oropharyngeal Candidiasis

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JAHANSHIRI Z.; RAZZAGHI ABYANEH M.

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Journal: Pathobiology Research Year:2020 | Volume:23 | Issue:1 Page(s): 1-9

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Information Journal Paper

Title

Comparison of Conventional and Molecular Methods in Identification of Candida Species Isolated from Oropharyngeal Candidiasis

Author(s)

JAHANSHIRI Z. | RAZZAGHI ABYANEH M. | Issue Writer Certificate

Keywords

CandidaQ2

Molecular TechniqueQ2

Conventional MethodsQ1

IdentificationQ2

Abstract

Aims Candida species are normal flora in the human body and are the main cause of hospital infections in people with underlying disease. The purpose of the present study was comparison of the conventional and molecular laboratory methods in identifying different species of Candida to find the optimal method. Materials & Methods 60 Candida isolates were obtained from oropharyngeal candidiasis patients and recognized using laboratory routine methods including germ tube production, culture on CHROMagar medium, API 20 C AUX kit and molecular ITS sequencing method. The concordances between the methods compared to the molecular method were measured by the kappa coefficient using SPSS 16. 0 software. Findings Out of 60 Candida isolates, 10 isolates (16. 6%) in germ tube formation test, 8 isolates (13. 33%) in the API test, and 9 isolates (15%) in the culture on CHROMagar medium have different results in comparison to the ITS sequencing method. Germ tube (k= 0. 90) and culture on CHROMagar medium methods (k= 0. 66) showed the highest and the lowest agreement with the molecular method in the Identification of Candida albicans species respectively. Conclusion The results showed that Conventional Methods alone cannot diagnose all Candida species and molecular methods such as ITS sequencing can be used to confirm the Identification.

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APA: Copy

JAHANSHIRI, Z., & RAZZAGHI ABYANEH, M.. (2020). Comparison of Conventional and Molecular Methods in Identification of Candida Species Isolated from Oropharyngeal Candidiasis. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), 23(1 ), 1-9. SID. https://sid.ir/paper/398441/en

Vancouver: Copy

JAHANSHIRI Z., RAZZAGHI ABYANEH M.. Comparison of Conventional and Molecular Methods in Identification of Candida Species Isolated from Oropharyngeal Candidiasis. PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES)[Internet]. 2020;23(1 ):1-9. Available from: https://sid.ir/paper/398441/en

IEEE: Copy

Z. JAHANSHIRI, and M. RAZZAGHI ABYANEH, “Comparison of Conventional and Molecular Methods in Identification of Candida Species Isolated from Oropharyngeal Candidiasis,” PATHOBIOLOGY RESEARCH (MODARES JOURNAL OF MEDICAL SCIENCES), vol. 23, no. 1 , pp. 1–9, 2020, [Online]. Available: https://sid.ir/paper/398441/en

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