TOXOPLASMOSIS INDIRECT IMMUNOFLUORESCENT ANTIBODY TEST AGREEMENT RATE AMONG FOUR LABORATORY CENTERS

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SARAEI SAHNEH SARAEI M.; JAHANI HASHEMI H.

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Journal: JOURNAL OF GUILAN UNIVERSITY OF MEDICAL SCIENCES Year:2008 | Volume:16 | Issue:64 Page(s): 51-59

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Title

TOXOPLASMOSIS INDIRECT IMMUNOFLUORESCENT ANTIBODY TEST AGREEMENT RATE AMONG FOUR LABORATORY CENTERS

Author(s)

SARAEI SAHNEH SARAEI M. | JAHANI HASHEMI H. | Issue Writer Certificate

Keywords

HEMATOLOGIC TESTSQ3

IMMUNO FLUORESCENT TECHNIQUEQ3

INDIRECTQ3

LABORATORY TECHNIQUES AND PROCEDURES TOXOPLASMOSISQ3

Abstract

Introduction: INDIRECT immunofluorescent antibody (IFA) test is one of the most common serologi61 tests for toxoplasmosis. Some of Toxoplasma seroepidemiological studies used this test in Iran. Review the studies which were performed by this test in different areas of the country with different climates, shows according to main way of transmission of Toxoplasma gondii in Iran, performing the test needed consideration. It seems that these probable contradictions are due to mistakes of the test procedure in some of the laboratories.Objective: This study was performed to determine the agreement rate of IFA toxoplasmosis test among 4 laboratory centers.Materials and Methods: The study is a multicenter evaluation. With the consideratiorof et hical values, serums were prepared from 100 subjects' who had referred for pre-marriage examinations to Qazvin medical community center in the year 2006. Each serum divided to four parts and in each laboratory was examined for specific IgG anti-Toxoplasma antibodies with the IFA test. The tests in each center were performed according to common protocol of that center. The sera in all laboratories were examined at 1:20, 1:100, 1:200 and above. The titration ³1:20 was considered as positive. The kappa coefficient between each 2 centers was calculated.Results: The seropositivity rates of IgG-IFA Toxoplasma test in the 4 centers were 72%, 44%, 34% and 23%. The kappa coefficient in comparing with 2 centers, for upper limit was 0.36 and for the lower limit was 0.16.Conclusion: In the present study, the agreement rates among the four centers were very lower than our expect it seems that, performing this test in some of laboratories in country accompanied with some defects that should be corrected. Therefore, we recommend to survey separately.

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APA: Copy

SARAEI SAHNEH SARAEI, M., & JAHANI HASHEMI, H.. (2008). TOXOPLASMOSIS INDIRECT IMMUNOFLUORESCENT ANTIBODY TEST AGREEMENT RATE AMONG FOUR LABORATORY CENTERS. JOURNAL OF GUILAN UNIVERSITY OF MEDICAL SCIENCES, 16(64), 51-59. SID. https://sid.ir/paper/39851/en

Vancouver: Copy

SARAEI SAHNEH SARAEI M., JAHANI HASHEMI H.. TOXOPLASMOSIS INDIRECT IMMUNOFLUORESCENT ANTIBODY TEST AGREEMENT RATE AMONG FOUR LABORATORY CENTERS. JOURNAL OF GUILAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2008;16(64):51-59. Available from: https://sid.ir/paper/39851/en

IEEE: Copy

M. SARAEI SAHNEH SARAEI, and H. JAHANI HASHEMI, “TOXOPLASMOSIS INDIRECT IMMUNOFLUORESCENT ANTIBODY TEST AGREEMENT RATE AMONG FOUR LABORATORY CENTERS,” JOURNAL OF GUILAN UNIVERSITY OF MEDICAL SCIENCES, vol. 16, no. 64, pp. 51–59, 2008, [Online]. Available: https://sid.ir/paper/39851/en

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