TRANSFECTION OF BMSCS BY PSEC-TAG-A-CNTF FOR SPINAL CORD INJURY

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ABBASZADEH HOJJATALLAH; TIRAHI TAKI; NOORI ZADEH ALI; SADEGHIZADE MAJID; AZIZZADEH DELSHAD ALIREZA; TAHERI TAHER; KAZEMI HADI

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Journal: DANESHVAR MEDICINE Year:2014 | Volume:21 | Issue:110 Page(s): 0-0

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Title

TRANSFECTION OF BMSCS BY PSEC-TAG-A-CNTF FOR SPINAL CORD INJURY

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Keywords

TRANSFECTIONQ1

SUBCLONINGQ2

CNTFQ2

BMSCSQ2

Abstract

Background and Objective: Ciliary neurotrophic factor (CNTF) was originally described as a trophic factor supporting the survival of ciliary ganglion neurons in vitro. CNTF can also promote the survival and differentiation of a variety of nerve cells including sensory, motor, autonomic neurons and oligodendrocytes. To enhance the neurotrophic effect of BMSCS, the CNTF-pSec-tag 2 /hygro A plasmid was constructed and transferred into BMSCS by lipofectamin.Materials and Methods: Plasmid CNTF-pSec-tag 2 /hygro A was constructed and verified by sequencing. BMSCS were transfected by lipofectamin. The transcription of the gene was evaluated by RT-PCR and real time PCR, and the expression of protein was evaluated by western blotting and ELISA.Results: CNTF-pSec-tag 2 /hygro A plasmid was correctly verified. After TRANSFECTION, the transcription of CNTF gene and the expression of CNTF protein were proven by RT-PCR, western blotting and ELISA.Conclusion: CNTF-pSec-tag 2 /hygro A plasmid was correctly constructed and BMSCS were successfully transfected by TRANSFECTION and CNTF-pSec-tag 2 /hygro A protein can be expressed well, which is a good foundation for future studies on the transplantation of gene-modified BMSCS to promote spinal cord regeneration.

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APA: Copy

ABBASZADEH, HOJJATALLAH, TIRAHI, TAKI, NOORI ZADEH, ALI, SADEGHIZADE, MAJID, AZIZZADEH DELSHAD, ALIREZA, TAHERI, TAHER, & KAZEMI, HADI. (2014). TRANSFECTION OF BMSCS BY PSEC-TAG-A-CNTF FOR SPINAL CORD INJURY. DANESHVAR MEDICINE, 21(110), 0-0. SID. https://sid.ir/paper/401344/en

Vancouver: Copy

ABBASZADEH HOJJATALLAH, TIRAHI TAKI, NOORI ZADEH ALI, SADEGHIZADE MAJID, AZIZZADEH DELSHAD ALIREZA, TAHERI TAHER, KAZEMI HADI. TRANSFECTION OF BMSCS BY PSEC-TAG-A-CNTF FOR SPINAL CORD INJURY. DANESHVAR MEDICINE[Internet]. 2014;21(110):0-0. Available from: https://sid.ir/paper/401344/en

IEEE: Copy

HOJJATALLAH ABBASZADEH, TAKI TIRAHI, ALI NOORI ZADEH, MAJID SADEGHIZADE, ALIREZA AZIZZADEH DELSHAD, TAHER TAHERI, and HADI KAZEMI, “TRANSFECTION OF BMSCS BY PSEC-TAG-A-CNTF FOR SPINAL CORD INJURY,” DANESHVAR MEDICINE, vol. 21, no. 110, pp. 0–0, 2014, [Online]. Available: https://sid.ir/paper/401344/en

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