COMPARATIVE STUDY OF SELECTIVE MEDIA FOR RECOVERY OF PATHPGENIC YERSINIA ENTEROCOLITICA AND YERSINIA ENTEROCOLITICA –LIKE SPECIES FROM DIRECT AND STOOL CULTURE

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BAHMANI N.; SOLTAN DALAL MOHAMMAD MAHDI

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Journal: JOURNAL OF GUILAN UNIVERSITY OF MEDICAL SCIENCES Year:2005 | Volume:14 | Issue:53 Page(s): 1-8

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Title

COMPARATIVE STUDY OF SELECTIVE MEDIA FOR RECOVERY OF PATHPGENIC YERSINIA ENTEROCOLITICA AND YERSINIA ENTEROCOLITICA –LIKE SPECIES FROM DIRECT AND STOOL CULTURE

Author(s)

BAHMANI N. | SOLTAN DALAL MOHAMMAD MAHDI | Issue Writer Certificate

Keywords

CULTURE MEDIAQ4

YERSINIAQ3

YERSINIA ENTEROCOLITQ4

Abstract

Introduction: YERSINIA is a gram negative bacteria and member of the family enterobactericeae. Pathogenic Y.enterocoliticace is significant cause of human disease in many parts of the world.Objective: Three objectives were followed in this study: 1) determined ability of different media in pure growth of YERSINIA strains, 2) determine the ability of different media in suppression of fecal flora and 3) assessing the ability of these medias in recovery of YERSINIA from experimentally inoculated stool specimens.Materials and methods: This study contains three phases that at the first phase 42 suspensions of pure YERSINIA strains inoculated to CIN agar, Mac conkey agar (MAc). Cellobiose, Arginia, lysine Agar (CAl), YERSINIA selective Agar (YSA), Hekton Entric Agar (HEk) and blood Agar (BA), then recovery rate was compared with BA. At the second phase 20 suspensions of stool specimens without diarrhea was inoculated on above medias and its suppression rate was compared with BA. At the third phase suspension of mixed stool from phase two was diluted with 10-2 and 10-7 and 31 YERSINIA stains and two dilution rate of 103 and 104 Cfu/ml was added and rate of YERSINIA was compared with CIN agar.Results: At the first phase, maximum mean growth of pure culture YERSINIA strains belonged to CIN (89.5%) & minimum growth was on Hek (45.7%). At the second phase, maximum suppression rate belonged to CIN (88.2%) & minimum was MAC (9%) and at the third phase maximum recovery rate was on CAL (47.2%) and minimum was on HEk(20.2% & 24.1%) and MAC(17.7% & 21.4%). Conclusion: Statistical calculation and Bonferroni test indicated that CIN agar compared with other four medias is a highly selective and differential medium with high sensitivity. Our results indicated that fecal flora decreases recovery of enteric pathogens from stool specially Y. enterocalitica and use of high selective medium in laboratory significantly increases isolation of Y.enterocolifica and Y.enterocolitica -like species from multibacteria specimens like stool.

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APA: Copy

BAHMANI, N., & SOLTAN DALAL, MOHAMMAD MAHDI. (2005). COMPARATIVE STUDY OF SELECTIVE MEDIA FOR RECOVERY OF PATHPGENIC YERSINIA ENTEROCOLITICA AND YERSINIA ENTEROCOLITICA –LIKE SPECIES FROM DIRECT AND STOOL CULTURE . JOURNAL OF GUILAN UNIVERSITY OF MEDICAL SCIENCES, 14(53), 1-8. SID. https://sid.ir/paper/40276/en

Vancouver: Copy

BAHMANI N., SOLTAN DALAL MOHAMMAD MAHDI. COMPARATIVE STUDY OF SELECTIVE MEDIA FOR RECOVERY OF PATHPGENIC YERSINIA ENTEROCOLITICA AND YERSINIA ENTEROCOLITICA –LIKE SPECIES FROM DIRECT AND STOOL CULTURE . JOURNAL OF GUILAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2005;14(53):1-8. Available from: https://sid.ir/paper/40276/en

IEEE: Copy

N. BAHMANI, and MOHAMMAD MAHDI SOLTAN DALAL, “COMPARATIVE STUDY OF SELECTIVE MEDIA FOR RECOVERY OF PATHPGENIC YERSINIA ENTEROCOLITICA AND YERSINIA ENTEROCOLITICA –LIKE SPECIES FROM DIRECT AND STOOL CULTURE ,” JOURNAL OF GUILAN UNIVERSITY OF MEDICAL SCIENCES, vol. 14, no. 53, pp. 1–8, 2005, [Online]. Available: https://sid.ir/paper/40276/en

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