DESIGNING & PRODUCING POLYTOPE DNA VACCINE CONTAINING HBSAG GENE FOR THE INDUCTION OF PROTECTIVE IMMUNITY AGAINST HEPATITIS C

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MEMARNEZHADIAN A.; ROUHVAND F.; MOTEVALI F.; AGHASADEGHI M.R.

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Journal: Feyz Medical Sciences Journal Year:2009 | Volume:13 | Issue:3 (SERIAL 51) Page(s): 163-173

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Title

DESIGNING & PRODUCING POLYTOPE DNA VACCINE CONTAINING HBSAG GENE FOR THE INDUCTION OF PROTECTIVE IMMUNITY AGAINST HEPATITIS C

Author(s)

MEMARNEZHADIAN A. | ROUHVAND F. | MOTEVALI F. | AGHASADEGHI M.R. | Issue Writer Certificate

Keywords

HEPATITIS CQ3

VACCINESQ3

DNAQ3

HEPATITIS B SURFACE ANTIGENQ3

HLA-A0201 ANTIGENQ2

EPITOPESQ2

Abstract

Background: Considering the immunosuppressive effects and prevalent mutations in some HCV antigens, induction of CD8+ T cell responses is focused on conserved and critical EPITOPES which as a multi-epitope vaccine can prevent the chronic nature of the disease. Materials and Methods: Two immunodominant HLA-A2-restricted human EPITOPES (E2614- 622 and NS31406-1415) and two H-2d-restricted mouse EPITOPES (core132-142 and E2405-414) were designed in a sequential tandem, predicted by immunoinformatic analyses. Following the synthesis, related nucleotide sequence was cloned into the pcDNA3.1 vector with and without the fusion of HEPATITIS B SURFACE ANTIGEN (HBsAg). Two constructed plasmids (pcDNA3.1.HPOL and pcDNA3.1.POL, respectively) were evaluated for the protein expression and secretion in Cos-7 cell line. After the vaccination of BALB/c mice (n=6 in each group) with different DNA and peptide immunization regimens, CD8+ T cell activity as well as the type and protective potency of the induced responses were evaluated. Results: Despite the induction of epitope-specific responses in pcDNA3.1.POL injected mice, the group immunized with pcDNA3.1.HPOL indicated a significant increase in the number and activity of CD8+ T cells (P<0.05). Peptide boosting of this group (formulated in two human-compatible adjuvant) still led to the more activation of CD8+ cells, induction of Th1 response and the inhibition of tumor model growth (P<0.05).Conclusion: Fusion of HBsAg as a particle-forming sequence and the source of helper EPITOPES along the DNA-prime/peptide-boosting immunization regimen are proposed as two promising strategies to improve the CTL multi-epitope VACCINES against HCV.

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APA: Copy

MEMARNEZHADIAN, A., ROUHVAND, F., MOTEVALI, F., & AGHASADEGHI, M.R.. (2009). DESIGNING & PRODUCING POLYTOPE DNA VACCINE CONTAINING HBSAG GENE FOR THE INDUCTION OF PROTECTIVE IMMUNITY AGAINST HEPATITIS C. FEYZ, 13(3 (SERIAL 51)), 163-173. SID. https://sid.ir/paper/402926/en

Vancouver: Copy

MEMARNEZHADIAN A., ROUHVAND F., MOTEVALI F., AGHASADEGHI M.R.. DESIGNING & PRODUCING POLYTOPE DNA VACCINE CONTAINING HBSAG GENE FOR THE INDUCTION OF PROTECTIVE IMMUNITY AGAINST HEPATITIS C. FEYZ[Internet]. 2009;13(3 (SERIAL 51)):163-173. Available from: https://sid.ir/paper/402926/en

IEEE: Copy

A. MEMARNEZHADIAN, F. ROUHVAND, F. MOTEVALI, and M.R. AGHASADEGHI, “DESIGNING & PRODUCING POLYTOPE DNA VACCINE CONTAINING HBSAG GENE FOR THE INDUCTION OF PROTECTIVE IMMUNITY AGAINST HEPATITIS C,” FEYZ, vol. 13, no. 3 (SERIAL 51), pp. 163–173, 2009, [Online]. Available: https://sid.ir/paper/402926/en

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