Molecular isolation of a human papilloma virus from blood serum in patients with leukemia in Kerman

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Ranjbar Zeidabadi H.; KHEIRKHAH B.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Journal: Feyz Medical Sciences Journal Year:2018 | Volume:22 | Issue:4 Page(s): 355-361

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Information Journal Paper

Title

Molecular isolation of a human papilloma virus from blood serum in patients with leukemia in Kerman

Author(s)

Ranjbar Zeidabadi H. | KHEIRKHAH B. | Issue Writer Certificate

Keywords

CancerQ2

Human papilloma virusQ2

CLLQ1

ALLQ1

Abstract

Background: ALL&fgrp=ALL&fyrs=ALL" target="_blank">Cancer is the second common cause of death in developed countries. Viruses are one of the most important environmental factors which increase the risk of developing ALL&fgrp=ALL&fyrs=ALL" target="_blank">Cancer. The aim of this study was to identify the ALL&fgrp=ALL&fyrs=ALL" target="_blank">Human papilloma virus (HPV) in patients with the blood ALL&fgrp=ALL&fyrs=ALL" target="_blank">Cancer. Materials and Methods: In this case-control study, blood serum samples were collected from 35 patients with Chronic lymphocytic leukemia (ALL&fgrp=ALL&fyrs=ALL" target="_blank">CLL) and 25 patients with Acute lymphoblastic leukemi (ALL) from Kerman medical centers. Thirty healthy individuals were considered as a control group. After DNA extraction from the serum samples, the polymerase chain reaction (PCR) was performed to detect HPV. Results: The specific sequence of HPV was observed in 15 samples (25%) of the patients’ serum. Out of this number, five samples (14. 25%) of the 35 patients had ALL&fgrp=ALL&fyrs=ALL" target="_blank">CLL and 10 samples (40%) of 25 patients had ALL. Also, no positive sample was found in the serum of the patients of the control group. Out of the 15 patients suffering from HPV, seven (46. 62%) and three (19. 98%) had HPV16 and HPV18, respectively and five samples (33. 4%) were of other types of HPV. Conclusion: This study showed that the PCR method with specific primers of Papilloma Virus 16 and 18 is a suitable and accurate method for detecting human papillomavirus.

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References

Prevalence of Mycoplasma hyorhinis contamination in tissues samples from cancer patients: A Brief Report

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APA: Copy

Ranjbar Zeidabadi, H., & KHEIRKHAH, B.. (2018). Molecular isolation of a human papilloma virus from blood serum in patients with leukemia in Kerman. FEYZ, 22(4 ), 355-361. SID. https://sid.ir/paper/40494/en

Vancouver: Copy

Ranjbar Zeidabadi H., KHEIRKHAH B.. Molecular isolation of a human papilloma virus from blood serum in patients with leukemia in Kerman. FEYZ[Internet]. 2018;22(4 ):355-361. Available from: https://sid.ir/paper/40494/en

IEEE: Copy

H. Ranjbar Zeidabadi, and B. KHEIRKHAH, “Molecular isolation of a human papilloma virus from blood serum in patients with leukemia in Kerman,” FEYZ, vol. 22, no. 4 , pp. 355–361, 2018, [Online]. Available: https://sid.ir/paper/40494/en

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