Evaluation of apoptosis related molecular profile in the hippocampus following microinjection of amyloid-beta into the rat’ s entorhinal cortex; Protective role of calcium channel blockers

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Gholami Pourbadie Hamid; Joneidi Marzieh; KHODAGHOLI FARIBA; SHAERZADEH FATEMEH

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Journal: IRANIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY Year:2019 | Volume:3 | Issue:2 (10) Page(s): 89-98

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Title

Evaluation of apoptosis related molecular profile in the hippocampus following microinjection of amyloid-beta into the rat’ s entorhinal cortex; Protective role of calcium channel blockers

Author(s)

Gholami Pourbadie Hamid | Joneidi Marzieh | KHODAGHOLI FARIBA | SHAERZADEH FATEMEH | Issue Writer Certificate

Keywords

Alzheimer's disease

entorhinal cortex

calcium channel blockers

CA1

Abstract

Background and aims: Alzheimer's disease (AD) is a progressive brain disorder that is associated with dementia. The entorhinal cortex (EC) is one of the first brain regions affected in AD. High level of beta-amyloid (Aβ ) is seen in various brain regions, including the EC. Previous studies have shown that Aβ causes calcium dyshomeostasis. In this study, molecular changes in the CA1 region following microinjection of Aβ into the EC and the potential protective role of calcium channel blockers was investigated. Methods: Aβ (1 μ g/2 μ l) was injected into the right EC of male Wistar rats under stereotaxic surgery, and then a guide cannula was planted in the right ventricle. Isradipine and nimodipine were intraventricularly injected at 30 μ g daily for 6 days. On the seventh day, the expression of Calpain 2, Caspase 12 and 3 in hippocampal CA1 was measured by western blot technique. Pro-apoptotic changes were also assessed by Tunnel test. Results: Results indicated that Aβ injection into the EC increased the expression of Calpain 2, Caspase 12 and 3 in the CA1 region. Apoptotic cells were also increased in the CA1 region following amyloidopathy in the EC. Following the treatment of the rats with isradipine and nimodipine, the expression of Calpain 2, Caspase 12 and 3 decreased, and the number of apoptotic cells was returned to the control level. Conclusion: EC amyloidopathy may change the molecular profile associated with apoptosis in neighbor regions such as CA1 and treatment with calcium channel blockers can prevent the changes.

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APA: Copy

Gholami Pourbadie, Hamid, Joneidi, Marzieh, KHODAGHOLI, FARIBA,. (2019). Evaluation of apoptosis related molecular profile in the hippocampus following microinjection of amyloid-beta into the rat’ s entorhinal cortex; Protective role of calcium channel blockers. IRANIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 3(2 (10) ), 89-98. SID. https://sid.ir/paper/405854/en

Vancouver: Copy

Gholami Pourbadie Hamid, Joneidi Marzieh, KHODAGHOLI FARIBA. Evaluation of apoptosis related molecular profile in the hippocampus following microinjection of amyloid-beta into the rat’ s entorhinal cortex; Protective role of calcium channel blockers. IRANIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY[Internet]. 2019;3(2 (10) ):89-98. Available from: https://sid.ir/paper/405854/en

IEEE: Copy

Hamid Gholami Pourbadie, Marzieh Joneidi, FARIBA KHODAGHOLI, an, “Evaluation of apoptosis related molecular profile in the hippocampus following microinjection of amyloid-beta into the rat’ s entorhinal cortex; Protective role of calcium channel blockers,” IRANIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, vol. 3, no. 2 (10) , pp. 89–98, 2019, [Online]. Available: https://sid.ir/paper/405854/en

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