EXTRACTION AND PURIFICATION OF THE CAPSULE OF STAPHYLOCOCCUS AUREUS TYPE 2

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

HAVAEI S.A.; NASERPOUR FARIVAR T.; SAFAARI MAHMOUD

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Feyz Medical Sciences Journal Year:2001 | Volume:5 | Issue:19 Page(s): 41-46

Download Full-Text

Persian Verion

View:

1,299

Download:

Cites:

Information Journal Paper

Title

EXTRACTION AND PURIFICATION OF THE CAPSULE OF STAPHYLOCOCCUS AUREUS TYPE 2

Author(s)

HAVAEI S.A. | NASERPOUR FARIVAR T. | SAFAARI MAHMOUD | Issue Writer Certificate

Keywords

STAPHYLOCOCCUS AUREUSQ2

POLYSACCHARIDE CAPSULEQ4

FINAL GROWTH PHASEQ4

PURIFIED ANTIGENQ4

EXTRACTIONQ4

Abstract

History and Objectives:Extraction and purification of POLYSACCHARIDE CAPSULE is the first step for preparation preventive vaccines in infections as a result of encapsulated STAPHYLOCOCCUS AUREUS. This study was carried out to extract and purify STAPHYLOCOCCUS AUREUS type 2.Materials and Methods:The descriptive and explorative strategy of this study was conducted on STAPHYLOCOCCUS AUREUS (Smith diffuse variant) in BID broth in a shaking incubator (200 rpm) in an aerobic conditions at a temperature of 37°C. The capsule was extracted using dilute acid and heating method. Then, dialysis was done overnight at 4°C and the product was lyophilized. Lyophilized powder was purified using a cellulose DEAE column (2.6 x 50 cm) by gradient method using bicarbonate ammonium buffers (0.01-1 M) at a velocity of 60 mt/h (Ion exchange chromatography). Finally, absorption spectrum was plotted and purified capsule was analyzed using scanning spectrophotometer.Results:Polysaccharide capsule was collected in FINAL GROWTH PHASE at 0.1 HCL and at a temperature of 100°C. Absorption spectrum was evaluated using H2S04/galacial acetic acid assay at wavelength range of 350-620 nm. The result showed that chromatographs are well matched with previously PURIFIED ANTIGENs. Conclusion and Recommendations:It is concluded that it is possible to extract and purify the capsule of Staphylococcus aurous type 2 and it is recommended to produce the related preventive vaccines.

Cites

No record.

References

No record.

Cite

APA: Copy

HAVAEI, S.A., NASERPOUR FARIVAR, T., & SAFAARI, MAHMOUD. (2001). EXTRACTION AND PURIFICATION OF THE CAPSULE OF STAPHYLOCOCCUS AUREUS TYPE 2. FEYZ, 5(19), 41-46. SID. https://sid.ir/paper/40686/en

Vancouver: Copy

HAVAEI S.A., NASERPOUR FARIVAR T., SAFAARI MAHMOUD. EXTRACTION AND PURIFICATION OF THE CAPSULE OF STAPHYLOCOCCUS AUREUS TYPE 2. FEYZ[Internet]. 2001;5(19):41-46. Available from: https://sid.ir/paper/40686/en

IEEE: Copy

S.A. HAVAEI, T. NASERPOUR FARIVAR, and MAHMOUD SAFAARI, “EXTRACTION AND PURIFICATION OF THE CAPSULE OF STAPHYLOCOCCUS AUREUS TYPE 2,” FEYZ, vol. 5, no. 19, pp. 41–46, 2001, [Online]. Available: https://sid.ir/paper/40686/en

Related Journal Papers