COMPARISON OF IN VITRO CULTURE OF PREANTRAL FOLLICLE ISOLATED FROM VITRIFIED WARMED MOUSE OVARIES WITH FRESH FOLLICLES IN CULTURE MEDIA

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MAZOUCHI T.; SALEHNIA M.; REZAZADEH VALOUJERDI M.; MOULA SEYED JAVAD; HAJIZADEH EBRAHIM

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Journal: Feyz Medical Sciences Journal Year:2007 | Volume:11 | Issue:1 (SERIAL 41) Page(s): 13-19

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Title

COMPARISON OF IN VITRO CULTURE OF PREANTRAL FOLLICLE ISOLATED FROM VITRIFIED WARMED MOUSE OVARIES WITH FRESH FOLLICLES IN CULTURE MEDIA

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VITRIFICATIONQ3

OVARYQ2

IN VITROQ3

FOLLICLEQ2

MICEQ2

Abstract

Background: VITRIFICATION is a simple and ultra rapid technique for the conservation of fertility. In this study, we compare the IN VITRO development of preantral FOLLICLEs obtained from fresh specimens with vitrified-warmed mouse ovaries.Materials and Methods: This experimental study was carried out on 20, 14-day-oldfemale MICE (NMRI). Ovaries were vitrified with a solution containing ethylene glycol, ficoll 70 and sucrose in PB1 (EGFS40) for 5 min, and transferred directly into liquid nitrogen and stored for one week. Fast warming was done by descending sucrose at room temperature.Preantral FOLLICLEs with 100-130 mm in diameter were mechanically isolated from fresh and vitrified-warmed ovaries and cultured in a-minimum essential medium (a-MEM) supplementedwith5% fetal bovine serum (FBS), 100 mIU/ml rFSH, 1%ITS, and 20ng/ml mrEGF IN VITRO for 10 days. Diameter of FOLLICLE, and, survival rate and number of antral FOLLICLEs in both groups were compared using t-test and chi-square test, respectively.Results: Isolated FOLLICLEs from the vitrified and no vitrified groups survived and grew IN VITRO culture. On the day 6 survival rates in the vitrified and fresh control groups were 72.1% and 78.6%, and, on the day 10, they were 66.9% and 72.6%, respectively. FOLLICLE antrum formation was 37.5% in the vitrified group while it was 43.5% in the fresh group in the 10th day. On the day 2, the mean diameters of fresh and vitrified FOLLICLEs were 158.11±11.23 and 155.48±8.35 and on the day 4, they were 201.56±9.87 and 193.42±8.46, respectively. There was no significant difference between the control and vitrified groups in these variables (p>0.05).Conclusion: Taken together, cry preservation of the OVARY by VITRIFICATION seems to be a promising method to preserve ovarian FOLLICLEs.

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APA: Copy

MAZOUCHI, T., SALEHNIA, M., REZAZADEH VALOUJERDI, M., MOULA, SEYED JAVAD, & HAJIZADEH, EBRAHIM. (2007). COMPARISON OF IN VITRO CULTURE OF PREANTRAL FOLLICLE ISOLATED FROM VITRIFIED WARMED MOUSE OVARIES WITH FRESH FOLLICLES IN CULTURE MEDIA. FEYZ, 11(1 (SERIAL 41)), 13-19. SID. https://sid.ir/paper/40853/en

Vancouver: Copy

MAZOUCHI T., SALEHNIA M., REZAZADEH VALOUJERDI M., MOULA SEYED JAVAD, HAJIZADEH EBRAHIM. COMPARISON OF IN VITRO CULTURE OF PREANTRAL FOLLICLE ISOLATED FROM VITRIFIED WARMED MOUSE OVARIES WITH FRESH FOLLICLES IN CULTURE MEDIA. FEYZ[Internet]. 2007;11(1 (SERIAL 41)):13-19. Available from: https://sid.ir/paper/40853/en

IEEE: Copy

T. MAZOUCHI, M. SALEHNIA, M. REZAZADEH VALOUJERDI, SEYED JAVAD MOULA, and EBRAHIM HAJIZADEH, “COMPARISON OF IN VITRO CULTURE OF PREANTRAL FOLLICLE ISOLATED FROM VITRIFIED WARMED MOUSE OVARIES WITH FRESH FOLLICLES IN CULTURE MEDIA,” FEYZ, vol. 11, no. 1 (SERIAL 41), pp. 13–19, 2007, [Online]. Available: https://sid.ir/paper/40853/en

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