Comparison of different techniques for loading doxorubicin in platelets microparticles

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DARBANDI A.; YARI F.; SHARIFI Z.; REZAIE N.

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Journal Paper

Paper Information

Journal: The Scientific Journal of Iranian Blood Transfusion Year:2021 | Volume:18 | Issue:1 Page(s): 60-69

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Title

Comparison of different techniques for loading doxorubicin in platelets microparticles

Author(s)

DARBANDI A. | YARI F. | SHARIFI Z. | REZAIE N. | Issue Writer Certificate

Keywords

DoxorubicinQ1

PlateletsQ1

Cell-derived microparticlesQ1

Abstract

Background and Objectives: Platelet microparticles are microvesicles derived from Platelets. Today, with the use of nanoparticles in cancer treatments, many limitations of traditional drug delivery methods are reduced. Doxorubicin, a chemotherapeutic drug available for the treatment of many cancers, has fluorescent properties and can be detected by fluorescent imaging in tissues. Aim of this study is to compare different drug loading methods into platelet microparticles. Materials and Methods: In this experimental study, the platelet concentrates were taken on their fifth day from Iranian Blood Transfusion Organization. Then platelet microparticles were extracted from those concentrated Platelets in several centrifugation stages. Fluorescent microbeads with onemicrometer size and anti-CD41/61 antibody were used to determine the size and identity of microparticles, respectively. Doxorubicin was loaded at 10 µ g/ml on platelet microparticles using three methods of incubation, cell-penetrating peptide, and Sonication. Using the autofluorescence property of Doxorubicin, the rate of drug loading on platelet microparticles was measured by flow cytometry method. Results: In terms of size, 95% of the total population of microparticles was less than one micrometer. The expression levels of CD41 and CD61 were 92. 39% and 80. 03%, respectively. The average fluorescence light intensities calculated in each of the incubation, sonication, and CPP methods were determined to be 79. 09% ± 11. 37, 47. 48% ± 25. 12, and 56. 69% ± 23. 24, respectively. Conclusions: As the incubation method has higher loading percentage, it could be an effective method for loading drug in platelet microparticles. Furthermore, the use of this method can be considered for clinical studies.

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APA: Copy

DARBANDI, A., YARI, F., SHARIFI, Z., & REZAIE, N.. (2021). Comparison of different techniques for loading doxorubicin in platelets microparticles. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 18(1 ), 60-69. SID. https://sid.ir/paper/412496/en

Vancouver: Copy

DARBANDI A., YARI F., SHARIFI Z., REZAIE N.. Comparison of different techniques for loading doxorubicin in platelets microparticles. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2021;18(1 ):60-69. Available from: https://sid.ir/paper/412496/en

IEEE: Copy

A. DARBANDI, F. YARI, Z. SHARIFI, and N. REZAIE, “Comparison of different techniques for loading doxorubicin in platelets microparticles,” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 18, no. 1 , pp. 60–69, 2021, [Online]. Available: https://sid.ir/paper/412496/en

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