Heterologous Expression, Purification and Determination of Activity of new Carboxypepetidase from Cohnella Ao1

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Naeemi Seyed Mahdi; AMINZADEH SAEED; SARI SOYAR; Nemati Fahimeh; NASEROLESLAMI MARYAM

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Journal: NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL Year:2021 | Volume:11 | Issue:42 Page(s): 95-109

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Title

Heterologous Expression, Purification and Determination of Activity of new Carboxypepetidase from Cohnella Ao1

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Keywords

CarboxypeptidaseQ2

CohnellaQ1

Folic AcidQ1

GlutamateQ1

IAU scienceQ1

Abstract

Aim and Background: Proteolytic enzymes are essential for the survival of living organisms by irreversible hydrolysis of peptide bond. Carboxypeptidases are proteolytic enzymes found in many organisms and are widely used in various industries, such as food and pharmaceuticals. Aims of this study are expression of Carboxypeptidase of chohnella A01, purification and determination of its activity. Materials and methods: The gene of Carboxypeptidase of Cohnella A01 has been cloned in pET26b(+) vector. Competent cells were prepared from E. coli BL21, plasmids were extracted and transformed to expression host. E. coli BL21 was cultured in LB medium and protein expression was induced by IPTG. After that cell harvested and recombinant protein was purified by Ni-TNA resin. Results: The expressed recombinant enzyme is a Glutamate Carboxypeptidase that capable of separating Glutamate from Folic Acid. Conclusion: Hence Carboxypeptidase G has medicinal application and recombinant Carboxypeptidase from Cohnella A01 is a thermostable enzyme, after optimization, can be use it for medicinal application.

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APA: Copy

Naeemi, Seyed Mahdi, AMINZADEH, SAEED, SARI, SOYAR, Nemati, Fahimeh, & NASEROLESLAMI, MARYAM. (2021). Heterologous Expression, Purification and Determination of Activity of new Carboxypepetidase from Cohnella Ao1. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, 11(42 ), 95-109. SID. https://sid.ir/paper/415195/en

Vancouver: Copy

Naeemi Seyed Mahdi, AMINZADEH SAEED, SARI SOYAR, Nemati Fahimeh, NASEROLESLAMI MARYAM. Heterologous Expression, Purification and Determination of Activity of new Carboxypepetidase from Cohnella Ao1. NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL[Internet]. 2021;11(42 ):95-109. Available from: https://sid.ir/paper/415195/en

IEEE: Copy

Seyed Mahdi Naeemi, SAEED AMINZADEH, SOYAR SARI, Fahimeh Nemati, and MARYAM NASEROLESLAMI, “Heterologous Expression, Purification and Determination of Activity of new Carboxypepetidase from Cohnella Ao1,” NEW CELLULAR & MOLECULAR BIOTECHNOLOGY JOURNAL, vol. 11, no. 42 , pp. 95–109, 2021, [Online]. Available: https://sid.ir/paper/415195/en

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