Effect of citric acid on p53 and p21 genes expression of the human gastric Adenocarcinoma cell line (AGS)

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Nasr Isfahani Hamed; ROUHI LEILA; Ziya Jahromi Noosha; Khashei Varnamkhasti Khalil

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Journal: Pars Journal of Medical Sciences Year:2021 | Volume:18 | Issue:4 Page(s): 17-24

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Title

Effect of citric acid on p53 and p21 genes expression of the human gastric Adenocarcinoma cell line (AGS)

Author(s)

Nasr Isfahani Hamed | ROUHI LEILA | Ziya Jahromi Noosha | Khashei Varnamkhasti Khalil | Issue Writer Certificate

Keywords

Citric AcidQ2

AdenocarcinomaQ3

Gene ExpressionQ2

p53Q2

p21Q1

Abstract

Introduction: Gastric Adenocarcinoma is the second leading cause of death from cancer worldwide. Citric Acid is a naturally organic acid that considered as a physiological inhibitor of enzymes involved in glycolysis pathway to remove cancer cells. In this study, the effect of Citric Acid on p53 and p21 genes expression of the human gastric Adenocarcinoma cell line (AGS) was examined. Materials and Methods: AGS cells with different concentrations of Citric Acid (400, 800 and 1600 μ g / ml) to examine the expression of genes p53 and p21 were incubated for 24 and 48 hours. Then, the expression of p53 and p21 genes were analyzed by Real Time PCR. Results: The results of this study indicate that treatment with Citric Acid increased the expression of p53 and p21 genes in the experimental groups compared to the control group. Over expression of p53 gene at concentrations of 1600 and 800 μ g / ml, respectively, was significant at 24 and 48 hours incubation times in dose and time independent manner. Expression of p21 gene at both incubation times was dose-and time dependent manner. Conclusion: The results indicate that Citric Acid can reduce the growth of gastric Adenocarcinoma cells by inducing expression of genes that involved in the apoptosis pathway and preventing proliferation. Therefore, Citric Acid seems to be used as an anticancer agent for the treatment of gastric cancer.

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APA: Copy

Nasr Isfahani, Hamed, ROUHI, LEILA, Ziya Jahromi, Noosha, & Khashei Varnamkhasti, Khalil. (2021). Effect of citric acid on p53 and p21 genes expression of the human gastric Adenocarcinoma cell line (AGS). PARS JOURNAL OF MEDICAL SCIENCES (JAHROM MEDICAL JOURNAL), 18(4 ), 17-24. SID. https://sid.ir/paper/415198/en

Vancouver: Copy

Nasr Isfahani Hamed, ROUHI LEILA, Ziya Jahromi Noosha, Khashei Varnamkhasti Khalil. Effect of citric acid on p53 and p21 genes expression of the human gastric Adenocarcinoma cell line (AGS). PARS JOURNAL OF MEDICAL SCIENCES (JAHROM MEDICAL JOURNAL)[Internet]. 2021;18(4 ):17-24. Available from: https://sid.ir/paper/415198/en

IEEE: Copy

Hamed Nasr Isfahani, LEILA ROUHI, Noosha Ziya Jahromi, and Khalil Khashei Varnamkhasti, “Effect of citric acid on p53 and p21 genes expression of the human gastric Adenocarcinoma cell line (AGS),” PARS JOURNAL OF MEDICAL SCIENCES (JAHROM MEDICAL JOURNAL), vol. 18, no. 4 , pp. 17–24, 2021, [Online]. Available: https://sid.ir/paper/415198/en

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