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Information Journal Paper

Title

Investigating the apoptotic and anti-proliferative effects of Juniperus excelsa fractions on Acute Lymphoblastic leukemia cell line (Nalm-6)

Pages

  276-286

Abstract

 Background and Objectives: Acute Lymphoblastic Leukemia is a hematological malignancy of lymphoid lineage. Side effects of chemotherapy drugs is a major reason for the tendency to use Herbal medicines. Previous studies on Juniperus excelsa (J. excelsa) have shown anti-cancer properties on some cancer cell lines. This study aimed to investigate the effect of three fractions of J. excelsa on Acute Lymphoblastic Leukemia cells (Nalm-6) and normal mononuclear cells (PBMC), and find a more toxic fraction. Materials and Methods: In this experimental study, the cytotoxic activity of these fractions in Nalm-6 was determined using Viability and MTT assay. By these tests, the more toxic fraction was selected and Apoptosis was assessed and confirmed with Annexin-V/propidium iodide staining by flow cytometry. Each test was repeated at least three times. Statistical analysis was assessed by oneway ANOVA and SPSS 23. Results: The results showed that concentrations of 2. 5 and 4 µ g/ml of chloroform and etherdopetrol fractions of J. excelsa, without considerable effect on PBMC, reduced significantly the cell viability and metabolic activity of Nalm-6 (p < 0. 01). Also, aquae fraction had no cytotoxic effect on Nalm-6 and normal cells. The more toxic fraction, which was chloroform fraction, was selected by these tests. According to the flow cytometry test, treated Nalm-6 cells with chloroform fraction demonstrated a 13-fold increase in the number of apoptotic cells compared with control (p < 0. 001). Conclusions: Our study showed that chloroform and etherdopetrol fractions have cytotoxic and antiproliferative effects on leukemic cells (Nalm-6) and induce Apoptosis.

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    APA: Copy

    Noorazar, L., ESMAEILI, S., MASHATI, P., GHAREHBAGHIAN, A., & SALARI, S.. (2021). Investigating the apoptotic and anti-proliferative effects of Juniperus excelsa fractions on Acute Lymphoblastic leukemia cell line (Nalm-6). THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 17(4 ), 276-286. SID. https://sid.ir/paper/415464/en

    Vancouver: Copy

    Noorazar L., ESMAEILI S., MASHATI P., GHAREHBAGHIAN A., SALARI S.. Investigating the apoptotic and anti-proliferative effects of Juniperus excelsa fractions on Acute Lymphoblastic leukemia cell line (Nalm-6). THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2021;17(4 ):276-286. Available from: https://sid.ir/paper/415464/en

    IEEE: Copy

    L. Noorazar, S. ESMAEILI, P. MASHATI, A. GHAREHBAGHIAN, and S. SALARI, “Investigating the apoptotic and anti-proliferative effects of Juniperus excelsa fractions on Acute Lymphoblastic leukemia cell line (Nalm-6),” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 17, no. 4 , pp. 276–286, 2021, [Online]. Available: https://sid.ir/paper/415464/en

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