Evaluation of Percentage of Interferon-Gamma Secreting T Helper Cells and Expression of Related Genes in Patients with Type 2 Diabetes Mellitus

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Sheikh Vida; Hoseini Aghdam Mirhamed; BEHZAD MAHDI

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Journal: Avicenna Journal of Clinical Medicine Year:2020 | Volume:27 | Issue:3 (SN 97) Page(s): 140-148

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Title

Evaluation of Percentage of Interferon-Gamma Secreting T Helper Cells and Expression of Related Genes in Patients with Type 2 Diabetes Mellitus

Author(s)

Sheikh Vida | Hoseini Aghdam Mirhamed | BEHZAD MAHDI | Issue Writer Certificate

Keywords

Diabetes Mellitus Type 2Q2

Interferon-GammaQ1

Transcription FactorsQ1

Abstract

Background and Objective: The alterations of the adaptive immune system are involved in type 2 diabetes mellitus (T2DM) pathogenesis. T helper 1 (Th1) cells or CD4+ T cells are the pro-inflammatory components of adaptive immunity with the main feature of Interferon-Gamma (IFN-γ ) secretion. The aim of this study was to evaluate the percentage of IFN-γ , assess the expression of related-genes in CD4+ T cells, including T-bet, IRF1, RUNX3, and NFκ B, and determine the correlation between them and clinical parameters in T2DM patients. Materials and Methods: In this case-control study, peripheral blood CD4 + T cells were isolated from 40 patients and 40 healthy controls (HCs). The percentage of IFN-γ secreting CD4 + T cells was assessed using flowcytometry, and the related-genes were evaluated using real-time polymerase chain reaction. Results: The percentage of IFN-γ secreting CD4+ T cells significantly increased in the patients in comparison to that of the HCs (P<0. 001). The expression levels of IRF1 and NFκ B were higher in the patients in comparison to those of the HCs (P=0. 02 and P<0. 001, respectively). A significant positive correlation between IFN-γ secreting CD4 + T cells and both IRF1 and NFκ B was observed in the patients (P=0. 001 and P=0. 002, respectively). There was a significant positive correlation between IFN-γ secreting CD4+ T cells, IRF1, and NFκ B with fasting plasma glucose and hemoglobin A1c in the patients (P<0. 001). Conclusion: Due to the increased response of Th1 cells (the production of IFN-γ and expression of related genes) in the patients and existing correlation between them and plasma glucose level, it seems that these inflammatory factors are involved in T2DM pathogenesis, and the use of IFN-γ pathway antagonists could be considered a novel therapeutic approach.

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APA: Copy

Sheikh, Vida, Hoseini Aghdam, Mirhamed, & BEHZAD, MAHDI. (2020). Evaluation of Percentage of Interferon-Gamma Secreting T Helper Cells and Expression of Related Genes in Patients with Type 2 Diabetes Mellitus. AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES), 27(3 (SN 97) ), 140-148. SID. https://sid.ir/paper/415810/en

Vancouver: Copy

Sheikh Vida, Hoseini Aghdam Mirhamed, BEHZAD MAHDI. Evaluation of Percentage of Interferon-Gamma Secreting T Helper Cells and Expression of Related Genes in Patients with Type 2 Diabetes Mellitus. AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES)[Internet]. 2020;27(3 (SN 97) ):140-148. Available from: https://sid.ir/paper/415810/en

IEEE: Copy

Vida Sheikh, Mirhamed Hoseini Aghdam, and MAHDI BEHZAD, “Evaluation of Percentage of Interferon-Gamma Secreting T Helper Cells and Expression of Related Genes in Patients with Type 2 Diabetes Mellitus,” AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES), vol. 27, no. 3 (SN 97) , pp. 140–148, 2020, [Online]. Available: https://sid.ir/paper/415810/en

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