Assessment of Cyclin E Gene Amplification as a Biomarker in Isfahan Province Breast Cancer Patients

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Torosian g.; SALEHI M.; HOUSHMAND M.

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Paper Information

Journal: JOURNAL OF ADVANCED BIOMEDICAL SCIENCES Year:2020 | Volume:10 | Issue:3 Page(s): 2625-2635

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Title

Assessment of Cyclin E Gene Amplification as a Biomarker in Isfahan Province Breast Cancer Patients

Author(s)

Torosian g. | SALEHI M. | HOUSHMAND M. | Issue Writer Certificate

Keywords

Breast cancerQ2

Cyclin EQ1

Real-Time PCRQ1

Gene amplificationQ2

BiomarkerQ1

Abstract

Background & Objective: Breast cancer is the most common cancer among women worldwide. Cellcycle is controlled by regulatory proteins called cyclins and Cyclin-Dependent Kinases. Aberrant activity of cyclins plays an important role in cancer development and progression. The Cyclin E gene, called CCNE1 or pCCNE1, is one of the four cyclins groups of the cell cycle, reported in a variety of cancers as well as Breast cancer, and is a notice target in cancer research. In this regard, Cyclin E Gene amplification in Breast cancer patients was evaluated and investigated and its capacity as a molecular Biomarker was assessed. Materials & Methods: Tumor tissue samples as well as adjacent healthy tissues, were excised and collected from Breast cancer patients undergoing surgery in Al-Zahra hospital. Genomic DNA was extracted using phenol-chloroform method. The extracted products concentration was normalized and applied in Real-Time PCR technique. Amplification of aforementioned gene was determined by calculating Cts using 2-Δ Δ Ct method and was evaluated by Wilcoxon statistical test. Results: The Cyclin E Gene amplification in Breast cancer tissue was significantly higher than healthy tissue (p = 0. 002). Furthermore, the amplification of Cyclin E gene was investigated as a Breast cancer Biomarker by the ROC curve statistical analysis. Conclusion: The results revealed, the area under the ROC curve (AUC) was close in 70 percent. As a result, the Cyclin E Gene amplification in neoplasm dramatically increases and can be used as a Biomarker for the diagnosis and prognosis of Breast cancer.

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APA: Copy

Torosian, g., SALEHI, M., & HOUSHMAND, M.. (2020). Assessment of Cyclin E Gene Amplification as a Biomarker in Isfahan Province Breast Cancer Patients. JOURNAL OF ADVANCED BIOMEDICAL SCIENCES, 10(3 ), 2625-2635. SID. https://sid.ir/paper/416311/en

Vancouver: Copy

Torosian g., SALEHI M., HOUSHMAND M.. Assessment of Cyclin E Gene Amplification as a Biomarker in Isfahan Province Breast Cancer Patients. JOURNAL OF ADVANCED BIOMEDICAL SCIENCES[Internet]. 2020;10(3 ):2625-2635. Available from: https://sid.ir/paper/416311/en

IEEE: Copy

g. Torosian, M. SALEHI, and M. HOUSHMAND, “Assessment of Cyclin E Gene Amplification as a Biomarker in Isfahan Province Breast Cancer Patients,” JOURNAL OF ADVANCED BIOMEDICAL SCIENCES, vol. 10, no. 3 , pp. 2625–2635, 2020, [Online]. Available: https://sid.ir/paper/416311/en

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