Neural Differentiation of Wharton's Jelly Stem Cells in Three-Dimensional Chitosan Based Culture Environment

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Mansoori E.; KERMANI SH.; ALIZADEH A.

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Journal: JOURNAL OF BABOL UNIVERSITY OF MEDICAL SCIENCES (JBUMS) Year:2019 | Volume:21 | Issue:1 Page(s): 266-272

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Title

Neural Differentiation of Wharton's Jelly Stem Cells in Three-Dimensional Chitosan Based Culture Environment

Author(s)

Mansoori E. | KERMANI SH. | ALIZADEH A. | Issue Writer Certificate

Keywords

Wharton's jellyQ1

Stem cellsQ2

Neural DifferentiationQ2

ChitosanQ1

Abstract

BACKGROUND AND OBJECTIVE: Wharton Jelly Stem cells (WJSCs) can be a good option for differentiating and regeneration of nervous system damage. Three dimensional (3D) cell cultures by providing a body-like environment have more advantages than the two dimensional (2D) cell cultures. The aim of this study was to investigate the Neural Differentiation of these cells in a 3D Chitosan based culture environment. METHODS: This experimental study was performed in 4 groups of 2&3D with and without differentiation media on WJSCs. First, to construct the hydrogel, hydroxyl ethyl cellulose was added to Chitosan-beta-glycerophosphate solution (8. 4: 0. 8) (HEC: CH-β-GF). Human WJSCs after isolation by enzymatic method from wartons' jelly of born infant in Imam Khomeini hospital in Tehran and characterization with flow cytometry, were cultured 5 10 5 cell in each well of 24-well plate in a 2D and 3D environment using the hydrogel in Neural Differentiation media for 4 days. Then, the Neural Differentiation of WJSCs was evaluated by quantitative analysis of β-Tubulin III, Nestin and β-actin (internal control) genes expression by Real Time PCR (RT-PCR). FINDINGS: The results of RT-PCR showed that expression of β-Tubulin III and Nestin genes in WJSCs was significantly increased by the influence of the Neural Differentiation media in both 2D (more than 4 folds)and 3D (more than 2 folds) culture conditions (p <0. 005). But the expression of β-Tubulin III and Nestin in 3D cell culture condition (more than 1. 5 folds) was greater than that in the 2D cell culture condition under the influence of the Neural Differentiation media (p <0. 01). CONCLUSION: The results showed that Neural Differentiation of WJSCs in a Chitosan based 3D environment is higher than 2D.

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APA: Copy

Mansoori, E., KERMANI, SH., & ALIZADEH, A.. (2019). Neural Differentiation of Wharton's Jelly Stem Cells in Three-Dimensional Chitosan Based Culture Environment. JOURNAL OF BABOL UNIVERSITY OF MEDICAL SCIENCES (JBUMS), 21(1 ), 266-272. SID. https://sid.ir/paper/416462/en

Vancouver: Copy

Mansoori E., KERMANI SH., ALIZADEH A.. Neural Differentiation of Wharton's Jelly Stem Cells in Three-Dimensional Chitosan Based Culture Environment. JOURNAL OF BABOL UNIVERSITY OF MEDICAL SCIENCES (JBUMS)[Internet]. 2019;21(1 ):266-272. Available from: https://sid.ir/paper/416462/en

IEEE: Copy

E. Mansoori, SH. KERMANI, and A. ALIZADEH, “Neural Differentiation of Wharton's Jelly Stem Cells in Three-Dimensional Chitosan Based Culture Environment,” JOURNAL OF BABOL UNIVERSITY OF MEDICAL SCIENCES (JBUMS), vol. 21, no. 1 , pp. 266–272, 2019, [Online]. Available: https://sid.ir/paper/416462/en

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