EVALUATION OF MODIFICATION IN M2 GENE OF INFLUENZA H1N1 VIRUS IN EXPRESSION IN BL21 E-COLI

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JAFARINEJAD FATEMEH; BANDEHPOUR MOJGAN; GHEFLAT SHIVA SADAT; KAZEMI BAHRAM

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Paper Information

Journal: Research in Medicine Year:2015 | Volume:39 | Issue:2 Page(s): 69-73

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Title

EVALUATION OF MODIFICATION IN M2 GENE OF INFLUENZA H1N1 VIRUS IN EXPRESSION IN BL21 E-COLI

Author(s)

JAFARINEJAD FATEMEH | BANDEHPOUR MOJGAN | GHEFLAT SHIVA SADAT | KAZEMI BAHRAM | Issue Writer Certificate

Keywords

CLONINGQ2

INFLUENZA VIRUSQ1

PROTEIN EXPRESSIONQ2

M2 PROTEINQ1

GENETIC MODIFICATIONQ2

PET22BQ2

Abstract

Background: This research was carried out for a by considering gene sequence and each amino acid codon in host, which express protein in order of probable vaccine production against influenza A, a major cause of mortality in a year. M2 PROTEIN is conserved and it can be a proper candidate vaccine for influenza infection.Materials and Methods: Present research was an experimental study. The sequence of M2 PROTEIN of Iran INFLUENZA VIRUS H1N1 strain was modified and synthesized. It was subcloned into PET22B vector recombinant protein and expressed in BL21 E-coli and confirmed with specific antibody by western blot technique.Results: The modified M2 gene was expressed in E-coli. The concentration of it was 300μg/ml and it was confirmed by western blotting with specific antibody.Conclusion: Influenza M2 PROTEIN with modification in sequence could be expressed in BL21 E-coli host.

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APA: Copy

JAFARINEJAD, FATEMEH, BANDEHPOUR, MOJGAN, GHEFLAT, SHIVA SADAT, & KAZEMI, BAHRAM. (2015). EVALUATION OF MODIFICATION IN M2 GENE OF INFLUENZA H1N1 VIRUS IN EXPRESSION IN BL21 E-COLI. PAJOUHESH DAR PEZESHKI, 39(2), 69-73. SID. https://sid.ir/paper/42101/en

Vancouver: Copy

JAFARINEJAD FATEMEH, BANDEHPOUR MOJGAN, GHEFLAT SHIVA SADAT, KAZEMI BAHRAM. EVALUATION OF MODIFICATION IN M2 GENE OF INFLUENZA H1N1 VIRUS IN EXPRESSION IN BL21 E-COLI. PAJOUHESH DAR PEZESHKI[Internet]. 2015;39(2):69-73. Available from: https://sid.ir/paper/42101/en

IEEE: Copy

FATEMEH JAFARINEJAD, MOJGAN BANDEHPOUR, SHIVA SADAT GHEFLAT, and BAHRAM KAZEMI, “EVALUATION OF MODIFICATION IN M2 GENE OF INFLUENZA H1N1 VIRUS IN EXPRESSION IN BL21 E-COLI,” PAJOUHESH DAR PEZESHKI, vol. 39, no. 2, pp. 69–73, 2015, [Online]. Available: https://sid.ir/paper/42101/en

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