INHIBITORY EFFECT OF ACIDULATED PHOSPHATE FLUORIDE AND SODIUM FLUORIDE GELS ON LEVELS OF CARIOGENIC ORGANISMS IN ORAL CAVITY USING QUANTITATIVE REAL TIME PCR

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HALI HALEH; JAHANIMOGHADAM FATEMEH; BAZRAFSHANI MOHAMMADREZA

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Paper Information

Journal: JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES Year:2016 | Volume:25 | Issue:134 Page(s): 105-113

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Title

INHIBITORY EFFECT OF ACIDULATED PHOSPHATE FLUORIDE AND SODIUM FLUORIDE GELS ON LEVELS OF CARIOGENIC ORGANISMS IN ORAL CAVITY USING QUANTITATIVE REAL TIME PCR

Author(s)

HALI HALEH | JAHANIMOGHADAM FATEMEH | BAZRAFSHANI MOHAMMADREZA | Issue Writer Certificate

Keywords

ACIDULATED PHOSPHATE FLUORIDEQ2

SODIUM FLUORIDEQ2

STREPTOCOCCUS MUTANSQ2

LACTOBACILLUSQ2

QUANTITATIVE REAL TIME PCRQ2

Abstract

Background and purpose: Several factors are involved in caries prevention in children. One of the most effective factors is the appropriate use of fluoride. Fluoride induces its main effects in caries prevention through antibacterial effects and topical contact with enamel. In this study the inhibitory effects of SODIUM FLUORIDE (NaF) and ACIDULATED PHOSPHATE FLUORIDE (APF) gels on cariogenic microorganisms concentrations (Streptococcus mutans and LACTOBACILLUS) were compared using Quantitative Real-Time PCR.Materials and methods: A single blind parallel clinical trial was performed in which plaque samples were investigated twice (before and after the use of fluoride). The plaque samples of 44 children were studied by QUANTITATIVE REAL TIME PCR and the number of bacteria (lactobacilli and STREPTOCOCCUS MUTANS) was counted in each sample. Then, APF gel (Fluoride 1.23% +0.98% phosphoric acid, pH 5.3) was randomly used for 22 children. All the teeth (primary and permanent) were covered with fluoride by applicator. For other children 2% NaF gel (neutral pH) were used. After the use of fluoride, the numbers of bacteria were measured again by quantitative real-time PCR. Finally, the variability of microorganisms before and after the intervention was compared. Mann-Whitney test was applied for data analysis using SPSS V.18.Results: Significant reductions were observed in numbers of STREPTOCOCCUS MUTANS and LACTOBACILLUS after consumption of APF-gel (P<0.05).Conclusion: The APF gel exhibited more caries-preventive effect compared to NaF gel and could be used to benefit children’s oral health.

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APA: Copy

HALI, HALEH, JAHANIMOGHADAM, FATEMEH, & BAZRAFSHANI, MOHAMMADREZA. (2016). INHIBITORY EFFECT OF ACIDULATED PHOSPHATE FLUORIDE AND SODIUM FLUORIDE GELS ON LEVELS OF CARIOGENIC ORGANISMS IN ORAL CAVITY USING QUANTITATIVE REAL TIME PCR. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, 25(134), 105-113. SID. https://sid.ir/paper/44358/en

Vancouver: Copy

HALI HALEH, JAHANIMOGHADAM FATEMEH, BAZRAFSHANI MOHAMMADREZA. INHIBITORY EFFECT OF ACIDULATED PHOSPHATE FLUORIDE AND SODIUM FLUORIDE GELS ON LEVELS OF CARIOGENIC ORGANISMS IN ORAL CAVITY USING QUANTITATIVE REAL TIME PCR. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2016;25(134):105-113. Available from: https://sid.ir/paper/44358/en

IEEE: Copy

HALEH HALI, FATEMEH JAHANIMOGHADAM, and MOHAMMADREZA BAZRAFSHANI, “INHIBITORY EFFECT OF ACIDULATED PHOSPHATE FLUORIDE AND SODIUM FLUORIDE GELS ON LEVELS OF CARIOGENIC ORGANISMS IN ORAL CAVITY USING QUANTITATIVE REAL TIME PCR,” JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, vol. 25, no. 134, pp. 105–113, 2016, [Online]. Available: https://sid.ir/paper/44358/en

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