EFFECT OF VITRIFICATION ON NUMBER AND SURVIVAL OF IN VITRO PRODUCED BOVINE INNER CELL BLASTOCYTES

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JASHNI H.K.; NASR ESPHAHANI M.H.; IMANI H.; BAHARVAND H.; MARDANI M.

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Journal: JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES Year:2004 | Volume:14 | Issue:42 Page(s): 1-10

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Title

EFFECT OF VITRIFICATION ON NUMBER AND SURVIVAL OF IN VITRO PRODUCED BOVINE INNER CELL BLASTOCYTES

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Keywords

BLASTOCYSTISQ3

BLASTOCYSRIS INFECTIONSQ4

Abstract

Background and purpose: Embryonic stem cells (ESC) can be produced by culturing blastocytes inner cell mass in a proper condition. ESC is used as research tool in producing transgenic animals and in the study of embryo development. Produced blastocytes can be vitrified and used in appropriate time. Aim of this study was to determine the mean number and survival rate of vitrified ICM, in order to use vitrified embryos in production of stem cells.Materials and methods: After in vitro maturation and fertilization of immature bovine oocytes, embryos were co-cultured in HamsF10 medium over Vero cells for 7 or 8 days at 30oc and 5% CO2. 500 blastocytes were selected and divided into two groups; case and control. The blastocytes of the case group were vitrified (Ethylene glycol 40%, Ficoll 18% and 0.3M sucrose). After thawing, embryos were cultured for 24hr and the expanded embryos, along with the blastocytes in the control group were isolated by immunosurgery method. Cell survivals were assessed by trypan blue staining.Results: The survival rate of ICM cells at the day 7 blastocytes in the control and vitrified groups were 96% and 83% respectively, and the average number of ICM cells were 17.4 ± 3 and 14±2 respectively. The survival rate of ICM cells from day 8th blastocytes in control and vitrified groups were 95% and 82% respectively. Mean number of ICM cells were 18.6±3.Conclusion: Survival rate and number of blastocyte inner cell mass in the control and vitrified groups on the day 7 and 8 were significantly different. In vitrified condition the survival rate and number of the cells declines significantly. Therefore it is not recommended to use vitrified blastocytes for production of the embryonic stern cells.

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APA: Copy

JASHNI, H.K., NASR ESPHAHANI, M.H., IMANI, H., BAHARVAND, H., & MARDANI, M.. (2004). EFFECT OF VITRIFICATION ON NUMBER AND SURVIVAL OF IN VITRO PRODUCED BOVINE INNER CELL BLASTOCYTES. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, 14(42), 1-10. SID. https://sid.ir/paper/44412/en

Vancouver: Copy

JASHNI H.K., NASR ESPHAHANI M.H., IMANI H., BAHARVAND H., MARDANI M.. EFFECT OF VITRIFICATION ON NUMBER AND SURVIVAL OF IN VITRO PRODUCED BOVINE INNER CELL BLASTOCYTES. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2004;14(42):1-10. Available from: https://sid.ir/paper/44412/en

IEEE: Copy

H.K. JASHNI, M.H. NASR ESPHAHANI, H. IMANI, H. BAHARVAND, and M. MARDANI, “EFFECT OF VITRIFICATION ON NUMBER AND SURVIVAL OF IN VITRO PRODUCED BOVINE INNER CELL BLASTOCYTES,” JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, vol. 14, no. 42, pp. 1–10, 2004, [Online]. Available: https://sid.ir/paper/44412/en

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