GENOTYPE IDENTIFICATION OF ECHINOCOCCUS GRANULOSUS FROM PARAFFIN-EMBEDDED TISSUES OF HYDATID CYSTS ISOLATED FROM HUMAN BY PCR-RFLP

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GHOLAMI SHIRZAD; SOSARAI MASOUD; FAKHAR MAHDI; SHARIF MAHDI; DARYANI AHMAD

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Journal: Journal of Mazandaran University of Medical Sciences Year:2011 | Volume:21 | Issue:85 Page(s): 10-19

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Title

GENOTYPE IDENTIFICATION OF ECHINOCOCCUS GRANULOSUS FROM PARAFFIN-EMBEDDED TISSUES OF HYDATID CYSTS ISOLATED FROM HUMAN BY PCR-RFLP

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Keywords

HUMAN HYDATID CYSTQ2

E. GRANULOSUSQ3

PCR-RFLPQ2

GENOTYPEQ2

Abstract

Background and purpose: Identifying the various GENOTYPEs of Echinococcus granulosus as the agent of hydatid cysts in endemic areas can influence the disease control programs, particularly in humans. Therefore, this study was conducted to identify the different GENOTYPEs of Echinococcus granulosus from paraffin-embedded tissues of hydatid cysts isolated from human by PCR-RFLP.Materials and methods: To identify the molecular characteristics of Echinococcus granulosus, tissue samples from 30 human patients infected with hydatid cysts were collected from hospitals across the province of Golestan. DNA was extracted and characterized by PCR-RFLP method. In this study, 3 restriction endonuclease enzymes were used.Results: PCR product obtained from amplification of Echinococcus granulosus rDNA-ITS1 from HUMAN HYDATID CYSTs showed two different patterns of DNA bands in human isolates. In spite of the difference between human isolates in the size of DNA bands (1000 base pairs), the use of BD1/4S and EGF1/EGR2 primers showed that these isolates are to some extent similar in the size of band (391 base pairs). PCR products by RFLP method showed a different pattern of GENOTYPE or strain with Taq1 restriction enzyme in human isolates. No change in the size of DNA bands were observed with Msp1 and Alu1 restriction enzyme in human isolates.Conclusion: Therefore, genotypic differences and similarities between the size of DNA bands of Echinococcus granulosus from human isolates with PCR-RFLP method indicated the occurrence of different GENOTYPEs of Echinococcus granulosus in different parts of Golestan Province.

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APA: Copy

GHOLAMI, SHIRZAD, SOSARAI, MASOUD, FAKHAR, MAHDI, SHARIF, MAHDI, & DARYANI, AHMAD. (2011). GENOTYPE IDENTIFICATION OF ECHINOCOCCUS GRANULOSUS FROM PARAFFIN-EMBEDDED TISSUES OF HYDATID CYSTS ISOLATED FROM HUMAN BY PCR-RFLP. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, 21(85), 10-19. SID. https://sid.ir/paper/44882/en

Vancouver: Copy

GHOLAMI SHIRZAD, SOSARAI MASOUD, FAKHAR MAHDI, SHARIF MAHDI, DARYANI AHMAD. GENOTYPE IDENTIFICATION OF ECHINOCOCCUS GRANULOSUS FROM PARAFFIN-EMBEDDED TISSUES OF HYDATID CYSTS ISOLATED FROM HUMAN BY PCR-RFLP. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2011;21(85):10-19. Available from: https://sid.ir/paper/44882/en

IEEE: Copy

SHIRZAD GHOLAMI, MASOUD SOSARAI, MAHDI FAKHAR, MAHDI SHARIF, and AHMAD DARYANI, “GENOTYPE IDENTIFICATION OF ECHINOCOCCUS GRANULOSUS FROM PARAFFIN-EMBEDDED TISSUES OF HYDATID CYSTS ISOLATED FROM HUMAN BY PCR-RFLP,” JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, vol. 21, no. 85, pp. 10–19, 2011, [Online]. Available: https://sid.ir/paper/44882/en

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