Effect of Trehalose on the Expression of Heat Shock Protein 70 Gene in PC12 Cells Treated with Hydrogen Peroxide

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NAZARI ROBATI MAHDIEH; Norouzi Akram; Nasouti Roya

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Journal: JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES Year:2019 | Volume:29 | Issue:172 Page(s): 1-9

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Title

Effect of Trehalose on the Expression of Heat Shock Protein 70 Gene in PC12 Cells Treated with Hydrogen Peroxide

Author(s)

NAZARI ROBATI MAHDIEH | Norouzi Akram | Nasouti Roya | Issue Writer Certificate

Keywords

trehaloseQ1

heat shock protein 70Q1

oxidative stressQ2

Abstract

Background and purpose: oxidative stress is implicated in the pathogenesis of various diseases, including neurological disorders. In such stressful conditions, heat shock protein 70 (HSP70) protects cells, thus its pharmacological induction can be protective. The disaccharide trehalose exhibits various beneficial effects, including antioxidative effect. However, its impact on HSP70 is not obvious. This study was conducted to investigate the effect of trehalose on HSP70 gene expression in rat pheochromocytoma PC12 cells treated with hydrogen peroxide. Materials and methods: PC12 cells were pretreated with trehalose (12. 5, 25, 50 mM) for 24 hours, and then were treated with hydrogen peroxide (100 μ M) for 24 hours. Cell viability was determined via MTT assay and HSP70 gene expression was measured using Real-time polymerase chain reaction. Results: trehalose pretreatment increased viability of PC12 cells exposed to hydrogen peroxide in a dose dependent manner. Furthermore, HSP70 gene expression upregulated in cells that were pretreated with trehalose. The highest gene expression was at 50 mM trehalose. Conclusion: Current study showed that terhalose protects PC12 cells against hydrogen peroxide by increasing the expression of HSP70 gene.

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APA: Copy

NAZARI ROBATI, MAHDIEH, Norouzi, Akram, & Nasouti, Roya. (2019). Effect of Trehalose on the Expression of Heat Shock Protein 70 Gene in PC12 Cells Treated with Hydrogen Peroxide. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, 29(172 ), 1-9. SID. https://sid.ir/paper/44944/en

Vancouver: Copy

NAZARI ROBATI MAHDIEH, Norouzi Akram, Nasouti Roya. Effect of Trehalose on the Expression of Heat Shock Protein 70 Gene in PC12 Cells Treated with Hydrogen Peroxide. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2019;29(172 ):1-9. Available from: https://sid.ir/paper/44944/en

IEEE: Copy

MAHDIEH NAZARI ROBATI, Akram Norouzi, and Roya Nasouti, “Effect of Trehalose on the Expression of Heat Shock Protein 70 Gene in PC12 Cells Treated with Hydrogen Peroxide,” JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, vol. 29, no. 172 , pp. 1–9, 2019, [Online]. Available: https://sid.ir/paper/44944/en

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