Evaluation of Carba NP Method for Rapid Detection of Pseudomonas aeruginosa Isolates Producing Carbapenemase Enzymes

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Beig Masoumeh; ARABESTANI MOHAMMAD REZA

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Journal: JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES Year:2019 | Volume:29 | Issue:172 Page(s): 10-21

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Title

Evaluation of Carba NP Method for Rapid Detection of Pseudomonas aeruginosa Isolates Producing Carbapenemase Enzymes

Author(s)

Beig Masoumeh | ARABESTANI MOHAMMAD REZA | Issue Writer Certificate

Keywords

Pseudomonas aeruginosaQ2

disk diffusion antibiotic testQ1

PCRQ2

CarbaNPQ1

Abstract

Background and purpose: Recently, increased resistance to carbapenem antibiotics among Pseudomonas aeruginosa has raised concerns. The Carba NP test is used for detection of carbapenemase types. This study aimed at applying Carba NP method for rapid detection of P. aeruginosa isolates producing carbapenemase enzymes. Materials and methods: A total of 97 clinical specimens was collected from patients in educational hospitals between November 2017 and May 2018 in Hamadan, Iran. Antibiotic susceptibility test was performed by disc diffusion method and MIC for imipenem was done by E-test. In order to detect carbapenemases enzymes, combined disk (CDT), CarbaNP, Modified Hodge (MHT), and Polymerase chain reaction (PCR) were performed. Statistical analysis was performed using SPSS V16. Results: The highest and lowest levels of resistance were found to be to Ceftriaxone 65 (67%) and Piperacilin/Tazobactam 38 (39. 2%), respectively. Among 97 clinical isolates of P. aeruginosa, 49 (50. 51%) were positive for carbapenemase genes by PCR test from which 48 (97. 95%) were positive for CarbaNP test. There were 48 (49. 48%) PCR negative isolates of which all (100%) showed negative results for CarbaNP test. Compared to PCR, the sensitivity and specificity of this test was 98% and 100%, respectively. Conclusion: This study showed that a high percentage of P. aeruginosa was resistant to Carbapenem antibiotics and the CarbaNP method was highly sensitive and specific for detection of carbapenemase enzymes.

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APA: Copy

Beig, Masoumeh, & ARABESTANI, MOHAMMAD REZA. (2019). Evaluation of Carba NP Method for Rapid Detection of Pseudomonas aeruginosa Isolates Producing Carbapenemase Enzymes. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, 29(172 ), 10-21. SID. https://sid.ir/paper/44956/en

Vancouver: Copy

Beig Masoumeh, ARABESTANI MOHAMMAD REZA. Evaluation of Carba NP Method for Rapid Detection of Pseudomonas aeruginosa Isolates Producing Carbapenemase Enzymes. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2019;29(172 ):10-21. Available from: https://sid.ir/paper/44956/en

IEEE: Copy

Masoumeh Beig, and MOHAMMAD REZA ARABESTANI, “Evaluation of Carba NP Method for Rapid Detection of Pseudomonas aeruginosa Isolates Producing Carbapenemase Enzymes,” JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, vol. 29, no. 172 , pp. 10–21, 2019, [Online]. Available: https://sid.ir/paper/44956/en

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