EVALUATION OF ICT, ELISA AND REAL TIME PCR IN DETECTION OF HEPATITIS B IN HEMODIALYSIS, THALASSEMIA AND HEMOPHILIC PATIENTS

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HABIBI AHMAD; ABEROMAND MOHAMMAD; MOHAMMADZADEH GHORBAN

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Journal: JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES Year:2016 | Volume:26 | Issue:143 Page(s): 37-47

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Title

EVALUATION OF ICT, ELISA AND REAL TIME PCR IN DETECTION OF HEPATITIS B IN HEMODIALYSIS, THALASSEMIA AND HEMOPHILIC PATIENTS

Author(s)

HABIBI AHMAD | ABEROMAND MOHAMMAD | MOHAMMADZADEH GHORBAN | Issue Writer Certificate

Keywords

HEPATITIS BQ1

REAL TIME PCRQ3

HEMODIALYSISQ3

THALASSEMIAQ3

HEMOPHILIAQ3

Abstract

Background and purpose: Recently, use of enzyme-linked immunosorbent assay (ELISA) and rapid Immunochromatographic test (ICT) in diagnosis and screening of patients with HEPATITIS B reduced the risk of hepatitis during blood transfusion. However, the incidence of HEPATITIS B is very high in highrisk patients such as HEMODIALYSIS, THALASSEMIA and HEMOPHILIA which receive a lot of blood transfusions.Also, due to the infection with mutant type of HEPATITIS B virus (HBV) and false negative results, using molecular tests such as polymerase chain reaction (PCR) are necessary for detection of HBV. In this study we aimed to compare the efficacy of ELISA and rapid ICT kits with gold standard REAL TIME PCR for the detection of HBV in high risk patients.Materials and methods: In a cross-sectional study, 100 patients consisting of HEMODIALYSIS, THALASSEMIA and HEMOPHILIA were assessed by REAL TIME PCR as the gold standard for detection of HBV DNA, fourth-generation ELISA kits including Dia-proÒ, PishtaztebÒ, PasteurÒ and ICT EcotestÒ were applied for detection of HBSAg.Results: Compared with REAL TIME PCR, sensitivity and specificity of fourth generation ELISA kits was 100% and positive predictive value (PPV) and negative predictive value (NPV) were 100%.Conclusion: Recent improvements in the monoclonal antibody production against mutant HEPATITIS B virus and their application in fourth-generation ELISA and rapid ICT kits resulted in significant increase in sensitivity and specificity of these methods. Thus, in emergency cases or lack of real-time PCR instruments, rapid ICT and ELISA kits could be suggest for detection of HBsAg.

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APA: Copy

HABIBI, AHMAD, ABEROMAND, MOHAMMAD, & MOHAMMADZADEH, GHORBAN. (2016). EVALUATION OF ICT, ELISA AND REAL TIME PCR IN DETECTION OF HEPATITIS B IN HEMODIALYSIS, THALASSEMIA AND HEMOPHILIC PATIENTS. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, 26(143), 37-47. SID. https://sid.ir/paper/45248/en

Vancouver: Copy

HABIBI AHMAD, ABEROMAND MOHAMMAD, MOHAMMADZADEH GHORBAN. EVALUATION OF ICT, ELISA AND REAL TIME PCR IN DETECTION OF HEPATITIS B IN HEMODIALYSIS, THALASSEMIA AND HEMOPHILIC PATIENTS. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2016;26(143):37-47. Available from: https://sid.ir/paper/45248/en

IEEE: Copy

AHMAD HABIBI, MOHAMMAD ABEROMAND, and GHORBAN MOHAMMADZADEH, “EVALUATION OF ICT, ELISA AND REAL TIME PCR IN DETECTION OF HEPATITIS B IN HEMODIALYSIS, THALASSEMIA AND HEMOPHILIC PATIENTS,” JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, vol. 26, no. 143, pp. 37–47, 2016, [Online]. Available: https://sid.ir/paper/45248/en

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