IN VITRO ANTI LEISHMANIAL EFFECT OF AGROSTEMMA GITHAGO EXTRACT ON LEISHMANIA MAJOR PROMASTIGOTES BY CELL COUNT AND MTT ASSAY

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NIAPOUR ALI; BOHLOOLI SHAHAB; SHARIFI PASANDI MARZIEH; MOHAMMADI GHALEHBIN BEHNAM

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Journal: Journal of Mazandaran University of Medical Sciences Year:2018 | Volume:28 | Issue:165 Page(s): 13-23

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Title

IN VITRO ANTI LEISHMANIAL EFFECT OF AGROSTEMMA GITHAGO EXTRACT ON LEISHMANIA MAJOR PROMASTIGOTES BY CELL COUNT AND MTT ASSAY

Author(s)

NIAPOUR ALI | BOHLOOLI SHAHAB | SHARIFI PASANDI MARZIEH | MOHAMMADI GHALEHBIN BEHNAM | Issue Writer Certificate

Keywords

LEISHMANIA MAJORQ2

AGROSTEMMA GITHAGOQ1

MTT ASSAYQ2

Abstract

Background and purpose: Treatment of leishmaniasis is getting complicated due to multipleside effects and drug resistance to first-line drugs. AGROSTEMMA GITHAGO is a plant with anti-cancer andcytocidal effects, so, this study was conducted to evaluate the anti leishmanial effect of its extract onLEISHMANIA MAJOR promastigotes by MTT ASSAY and cell count. Materials and methods: A total of 2. 5×106 LEISHMANIA MAJOR promastigotes in their stationaryphase were plated to each well of the 96 well culture plates. Cells were then incubated with increasingconcentrations of AGROSTEMMA GITHAGO extract (0. 05 – 2. 4 mg/ml) for 48 hours at 25° C. Glucantim wasused as standard control. Then, the supernatants were discarded and 50 μ l of MTT were added for 3hours. After centrifuge, the supernatants were replaced by 100 μ l of DMSO. The plate was read byELISA reader at 570 nm. Trypan blue staining was also performed to evaluate the effect of Agrostemmagithago extract on LEISHMANIA MAJOR promastigotes. Results: MTT ASSAY showed that increasing concentrations of Agrostemma extract couldsignificantly reduce cell viability of LEISHMANIA MAJOR promastigots in a dose dependent manner(p<0. 05). IC50 of the Agrostemma and Glucantime were 0. 365 and 71. 01 mg/ml, respectively. Conclusion: Aqueous extract of AGROSTEMMA GITHAGO was found to have stronger inhibitoryeffect than Glucantim on LEISHMANIA MAJOR promastigots.

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APA: Copy

NIAPOUR, ALI, BOHLOOLI, SHAHAB, SHARIFI PASANDI, MARZIEH, & MOHAMMADI GHALEHBIN, BEHNAM. (2018). IN VITRO ANTI LEISHMANIAL EFFECT OF AGROSTEMMA GITHAGO EXTRACT ON LEISHMANIA MAJOR PROMASTIGOTES BY CELL COUNT AND MTT ASSAY. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, 28(165 ), 13-23. SID. https://sid.ir/paper/45354/en

Vancouver: Copy

NIAPOUR ALI, BOHLOOLI SHAHAB, SHARIFI PASANDI MARZIEH, MOHAMMADI GHALEHBIN BEHNAM. IN VITRO ANTI LEISHMANIAL EFFECT OF AGROSTEMMA GITHAGO EXTRACT ON LEISHMANIA MAJOR PROMASTIGOTES BY CELL COUNT AND MTT ASSAY. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2018;28(165 ):13-23. Available from: https://sid.ir/paper/45354/en

IEEE: Copy

ALI NIAPOUR, SHAHAB BOHLOOLI, MARZIEH SHARIFI PASANDI, and BEHNAM MOHAMMADI GHALEHBIN, “IN VITRO ANTI LEISHMANIAL EFFECT OF AGROSTEMMA GITHAGO EXTRACT ON LEISHMANIA MAJOR PROMASTIGOTES BY CELL COUNT AND MTT ASSAY,” JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, vol. 28, no. 165 , pp. 13–23, 2018, [Online]. Available: https://sid.ir/paper/45354/en

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