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Information Journal Paper

Title

INVESTIGATION OF IMMOBILIZED LACCASE ENZYME ON NANOPOROUS SILICA APPLICATION FOR REMOVAL OF 2,4-DINITROPHENOL FROM AQUEOUS SOLUTION

Pages

  301-313

Abstract

 Background and purpose: 2, 4-dinitrophenol (2,4-DNP) is categorized as one of the priority pollutants that is used in pharmaceutical and chemical industries, pesticides, etc. In this study we aimed at investigating the BIODEGRADATION of 2,4-DNP by immobilized LACCASE on NANO-POROUS SILICA beads.Materials and methods: An experimental study was conducted in which the enzyme was chemically cross-linked on the NANO-POROUS SILICA beads. Temperature (40-60oC), contact time (2-12 h), mediator concentration (1-3 mM), pH (4-6) and initial pollutant concentration (10-30 ppm) were considered. The high performance liquid chromatography was applied to measure the degradation of the pollutant.Results: The maximum removal of 2,4-DNP (91%) was achieved at T=50oC, 2,4-DNP concentration of 10 ppm, mediator concentration of 1 mM, pH=5 and 12h contact time. ANOVA analysis showed  that contact time and pH had the most and lowest effect on the process efficiency, respectively.Conclusion: 2,4-DNP was effectively degraded by LACCASE. The reusability and resistibility of the enzyme improved through immobilization. The immobilized LACCASE on NANO-POROUS SILICA beads could be applied to remove a wide range of phenolic pollutants.

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    APA: Copy

    JONIDI JAFARI, AHMAD, REZAEI KALANTARY, ROSHANAK, DEHGHANIFARD, EMAD, MAHVI, AMIR HOSSEIN, FARAMARZI, MOHAMMDALI, & MOHAMMADIKALHORI, EBRAHIM. (2017). INVESTIGATION OF IMMOBILIZED LACCASE ENZYME ON NANOPOROUS SILICA APPLICATION FOR REMOVAL OF 2,4-DINITROPHENOL FROM AQUEOUS SOLUTION. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, 26(144), 301-313. SID. https://sid.ir/paper/45391/en

    Vancouver: Copy

    JONIDI JAFARI AHMAD, REZAEI KALANTARY ROSHANAK, DEHGHANIFARD EMAD, MAHVI AMIR HOSSEIN, FARAMARZI MOHAMMDALI, MOHAMMADIKALHORI EBRAHIM. INVESTIGATION OF IMMOBILIZED LACCASE ENZYME ON NANOPOROUS SILICA APPLICATION FOR REMOVAL OF 2,4-DINITROPHENOL FROM AQUEOUS SOLUTION. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2017;26(144):301-313. Available from: https://sid.ir/paper/45391/en

    IEEE: Copy

    AHMAD JONIDI JAFARI, ROSHANAK REZAEI KALANTARY, EMAD DEHGHANIFARD, AMIR HOSSEIN MAHVI, MOHAMMDALI FARAMARZI, and EBRAHIM MOHAMMADIKALHORI, “INVESTIGATION OF IMMOBILIZED LACCASE ENZYME ON NANOPOROUS SILICA APPLICATION FOR REMOVAL OF 2,4-DINITROPHENOL FROM AQUEOUS SOLUTION,” JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, vol. 26, no. 144, pp. 301–313, 2017, [Online]. Available: https://sid.ir/paper/45391/en

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