MOLECULAR STUDY OF PLASMID GENES AMPC OF ACINETOBACTER BAUMANNII ISOLATED FROM CLINICAL CASES USING MULTIPLEX PCR

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FEKRI SAJAD; SOLTANI BANAVANDI MOHAMMAD JAVAD; AMINI KUMARSS

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Journal: JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES Year:2017 | Volume:27 | Issue:155 Page(s): 157-162

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Title

MOLECULAR STUDY OF PLASMID GENES AMPC OF ACINETOBACTER BAUMANNII ISOLATED FROM CLINICAL CASES USING MULTIPLEX PCR

Author(s)

FEKRI SAJAD | SOLTANI BANAVANDI MOHAMMAD JAVAD | AMINI KUMARSS | Issue Writer Certificate

Keywords

ACINETOBACTER BAUMANNIIQ1

ANTIBIOTIC RESISTANCEQ2

AMPCQ1

MULTIPLEX PCRQ1

Abstract

Background and purpose: ACINETOBACTER BAUMANNII is an opportunistic pathogen that remains persistent in the environment for a long time. The eradication of this bacteria is difficult, since it has the ability to acquire ANTIBIOTIC RESISTANCE. The aims of this study were to identify theampC plasmid-mediated genes and antibiotic sensitivity ofACINETOBACTER BAUMANNII strains.Materials and methods: Sixty strains of ACINETOBACTER BAUMANNII isolates from wound, urine, blood, and respiratory secretions were used. The presence ofampC plasmid-mediated genes including FOX, MOX, DHA, ACC and CIT were evaluated by MULTIPLEX PCR. Antimicrobial susceptibility testing against 5 antibiotics was performed for all isolates.Results: MULTIPLEX PCR results showed that 39 samples (65%) had the CIT gene, 36 (60%) had theDHA gene, 12 (20%) had the MOX gene and 8 samples (13.3%) harboured ACC and FOX genes.Based on antibiogram results, the highest rate of resistance was found to cefepime (95%) and the highest rate of sensitivity was to gentamicin (45%).Conclusion: Current study showed a high percentage of AMPC plasmid-mediated genes and also high prevalence of ANTIBIOTIC RESISTANCE inACINETOBACTER BAUMANNII.

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APA: Copy

FEKRI, SAJAD, SOLTANI BANAVANDI, MOHAMMAD JAVAD, & AMINI, KUMARSS. (2017). MOLECULAR STUDY OF PLASMID GENES AMPC OF ACINETOBACTER BAUMANNII ISOLATED FROM CLINICAL CASES USING MULTIPLEX PCR. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, 27(155 ), 157-162. SID. https://sid.ir/paper/45502/en

Vancouver: Copy

FEKRI SAJAD, SOLTANI BANAVANDI MOHAMMAD JAVAD, AMINI KUMARSS. MOLECULAR STUDY OF PLASMID GENES AMPC OF ACINETOBACTER BAUMANNII ISOLATED FROM CLINICAL CASES USING MULTIPLEX PCR. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2017;27(155 ):157-162. Available from: https://sid.ir/paper/45502/en

IEEE: Copy

SAJAD FEKRI, MOHAMMAD JAVAD SOLTANI BANAVANDI, and KUMARSS AMINI, “MOLECULAR STUDY OF PLASMID GENES AMPC OF ACINETOBACTER BAUMANNII ISOLATED FROM CLINICAL CASES USING MULTIPLEX PCR,” JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, vol. 27, no. 155 , pp. 157–162, 2017, [Online]. Available: https://sid.ir/paper/45502/en

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