Detection of Ureaplasma urealyticum in Clinical Semen Samples in Infertile Men Using Molecular Method

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Tohidpour Maryam; Shahhosseiny Mohammadhassan; MEHRABIAN SEDIGHEH; SAREMI ABOTALEB

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES Year:2019 | Volume:28 | Issue:168 Page(s): 10-21

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

669

Download:

Cites:

Information Journal Paper

Title

Detection of Ureaplasma urealyticum in Clinical Semen Samples in Infertile Men Using Molecular Method

Author(s)

Tohidpour Maryam | Shahhosseiny Mohammadhassan | MEHRABIAN SEDIGHEH | SAREMI ABOTALEB | Issue Writer Certificate

Keywords

Ureaplasma urealyticumQ2

infertile menQ2

semenQ2

molecular methodQ1

Abstract

Background and purpose: Ureaplasma urealyticum is one of the most common causes of Non-Gonococcal Urethritis (NGU). Asymptomatic clinical infection caused by this bacterium can cause malnutrition of the sexual attachment glands and its presence in semen contributes to lower fertility. The aim of this study was to identify Ureaplasma urealyticum in semen of infertile men using PCR method as an accurate diagnostic method. Materials and methods The PCR test was optimized by standard strain to detect Ureaplasma urealyticum and then was studied in terms of specificity and limit of detection (LOD). semen samples were collected from 100 infertile men and each sample was divided into two parts: the first part was tested by semen analysis and the second part was tested by PCR method. DNA was extracted using phenol-chloroform method and the PCR test was done for detection of Ureaplasma urealyticum. Results Among the semen samples, 16 cases (16%) were found to be positive for Ureaplasma urealyticum. According to the spermogram test, the leukocyte level was also more than normal level in these samples (0-1 Mil/ml). Conclusion Screening of infertile couples for Ureaplasma urealyticum infection in those without clinical symptoms, thereby performing timely antibiotic therapy play key roles in treatment of infertility. Hence, PCR method is introduced as a valuable and reliable technique to identify Ureaplasma urealyticum.

Cites

No record.

References

Cite

APA: Copy

Tohidpour, Maryam, Shahhosseiny, Mohammadhassan, MEHRABIAN, SEDIGHEH, & SAREMI, ABOTALEB. (2019). Detection of Ureaplasma urealyticum in Clinical Semen Samples in Infertile Men Using Molecular Method. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, 28(168 ), 10-21. SID. https://sid.ir/paper/45588/en

Vancouver: Copy

Tohidpour Maryam, Shahhosseiny Mohammadhassan, MEHRABIAN SEDIGHEH, SAREMI ABOTALEB. Detection of Ureaplasma urealyticum in Clinical Semen Samples in Infertile Men Using Molecular Method. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2019;28(168 ):10-21. Available from: https://sid.ir/paper/45588/en

IEEE: Copy

Maryam Tohidpour, Mohammadhassan Shahhosseiny, SEDIGHEH MEHRABIAN, and ABOTALEB SAREMI, “Detection of Ureaplasma urealyticum in Clinical Semen Samples in Infertile Men Using Molecular Method,” JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, vol. 28, no. 168 , pp. 10–21, 2019, [Online]. Available: https://sid.ir/paper/45588/en

Related Journal Papers