OPTIMIZATION OF REFOLDING OF HUMAN VASCULAR ENDOTHELIAL GROWTH FACTOR-A121

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KAZEMI LOMEDASHT FATEMEH; BEHDANI MAHDI; POOSHANG BAGHERI KAMRAN; SHAHBAZZADEH DELAVAR; HABIBI ANBOUHI MAHDI

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Journal: MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES Year:2018 | Volume:40 | Issue:1 Page(s): 60-65

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Title

OPTIMIZATION OF REFOLDING OF HUMAN VASCULAR ENDOTHELIAL GROWTH FACTOR-A121

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Keywords

VASCULAR ENDOTHELIAL GROWTH FACTORQ1

ANGIOGENESISQ2

REFOLDINGQ1

Abstract

Background: VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) plays an important role in development of new blood vessel and ANGIOGENESIS. Human VEGF121 is smallest member of VEGF family. Production of active and correct form of VEGF is the most challenging issue. Methods: Here we described a method for optimization of REFOLDING of VEGF121 which was expressed in bacterial host. Enzyme linked immunosorbent assay and proliferation assay of human endothelial cells was performed to monitor REFOLDING and functional assay of VEGF121. Results: Using described method; VEGF was in correct fold and detected by antibody in ELISA. Furthermore, VEGF stimulated proliferation of human endothelial cells in dose-dependent manner. Conclusion: Refolded VEGF has potential for stem cell differentiation.

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APA: Copy

KAZEMI LOMEDASHT, FATEMEH, BEHDANI, MAHDI, POOSHANG BAGHERI, KAMRAN, SHAHBAZZADEH, DELAVAR, & HABIBI ANBOUHI, MAHDI. (2018). OPTIMIZATION OF REFOLDING OF HUMAN VASCULAR ENDOTHELIAL GROWTH FACTOR-A121. MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES, 40(1 ), 60-65. SID. https://sid.ir/paper/46896/en

Vancouver: Copy

KAZEMI LOMEDASHT FATEMEH, BEHDANI MAHDI, POOSHANG BAGHERI KAMRAN, SHAHBAZZADEH DELAVAR, HABIBI ANBOUHI MAHDI. OPTIMIZATION OF REFOLDING OF HUMAN VASCULAR ENDOTHELIAL GROWTH FACTOR-A121. MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2018;40(1 ):60-65. Available from: https://sid.ir/paper/46896/en

IEEE: Copy

FATEMEH KAZEMI LOMEDASHT, MAHDI BEHDANI, KAMRAN POOSHANG BAGHERI, DELAVAR SHAHBAZZADEH, and MAHDI HABIBI ANBOUHI, “OPTIMIZATION OF REFOLDING OF HUMAN VASCULAR ENDOTHELIAL GROWTH FACTOR-A121,” MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES, vol. 40, no. 1 , pp. 60–65, 2018, [Online]. Available: https://sid.ir/paper/46896/en

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