DETECTION OF MUTAGENIC EFFECTS OF MUSTARD GAS METABOLITES IN URINE OF RABBITS USING MICRO-TITRE FLUCTUATION TEST

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MOHADDES S.M.

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Paper Information

Journal: MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES Year:2000 | Volume:34 | Issue:45 Page(s): 93-98

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Title

DETECTION OF MUTAGENIC EFFECTS OF MUSTARD GAS METABOLITES IN URINE OF RABBITS USING MICRO-TITRE FLUCTUATION TEST

Author(s)

MOHADDES S.M. | Issue Writer Certificate

Keywords

SUFUR MUSTARDQ3

METABOLITEQ3

FLUCTUATION TESTQ2

MUTAGENIC EFFECTQ4

Abstract

The present study demonstrates the detection of MUTAGENIC EFFECTs of mustard gas METABOLITEs in urine samples of rabbits which were used as experimental animals in this study. We used micro titer FLUCTUATION TEST and Salmonella typhimurium (TA102) as bacterial tester strain. Three groups of rabbits were exposed to 0.8, 1.6 or 3.2 mg/kg of sulfur mustard, diluted in dimethyle suffixed (DMSO). The fourth group was received only DMSO and considered as control group. The urine samples were collected from all animals at 3 periods after treatment (3, 6 and 9 days) and assayeq for the presence of the mutagenic agents after ether and chloroform extraction.The results indicate that the samples collected at periods of 3 and 6 days exhibit significant mutagenic activity, while the samples collected in the period of 9 days were not significantly mutagenic. It was also concluded that FLUCTUATION TEST is a useful procedure for quick detection of the most hazardous METABOLITEs of the chemical agents.

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APA: Copy

MOHADDES, S.M.. (2000). DETECTION OF MUTAGENIC EFFECTS OF MUSTARD GAS METABOLITES IN URINE OF RABBITS USING MICRO-TITRE FLUCTUATION TEST. MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES, 34(45), 93-98. SID. https://sid.ir/paper/46936/en

Vancouver: Copy

MOHADDES S.M.. DETECTION OF MUTAGENIC EFFECTS OF MUSTARD GAS METABOLITES IN URINE OF RABBITS USING MICRO-TITRE FLUCTUATION TEST. MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2000;34(45):93-98. Available from: https://sid.ir/paper/46936/en

IEEE: Copy

S.M. MOHADDES, “DETECTION OF MUTAGENIC EFFECTS OF MUSTARD GAS METABOLITES IN URINE OF RABBITS USING MICRO-TITRE FLUCTUATION TEST,” MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES, vol. 34, no. 45, pp. 93–98, 2000, [Online]. Available: https://sid.ir/paper/46936/en

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