GINGER ACCELERATES GLUT4 TRANSLOCATION TO THE CELL MEMBRANE OF C2C12 MYOTUBES

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Tajik Kord Marjan; POORRAJAB FATEMEH; MOHITI ARDEKANI JAVAD; Azari Manizheh; Raeissi Alireza

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES Year:2016 | Volume:38 | Issue:3 Page(s): 34-41

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

2,310

Download:

Cites:

Information Journal Paper

Title

GINGER ACCELERATES GLUT4 TRANSLOCATION TO THE CELL MEMBRANE OF C2C12 MYOTUBES

Author(s)

Keywords

GINGERQ1

TYPE 2 DIABETES MELLITUSQ2

GLUT4 PROTEINQ1

MYOBLASTSQ1

Abstract

Backgrounds and Objectives: Diabetes type 2 is a metabolic disorder that affects many organs through chronic high blood levels of glucose. The GLUT4 translocation from cytosolic component to the cell membrane is the most important mechanism and strategy to compensate this situation. At the present study, we aimed to investigate the molecular mechanism of anti-diabetic and hypoglycemic effects of GINGER, through evaluating the translocation of GLUT4 in C2C12 myotubes.Material and Methods: In an experimental study, the C2C12 cells were treated with 50 mgr/mL concentration of ethyl acetate GINGER extract for 3 hours. Sub-cellular fractions were made by centrifugation from homogenized myotubes. After preparation of cytosolic and membrane fractions, the amount of GLUT-4 (an important glucose transporter) was determined using sodium dodecyl sulfate polyacrylamide gel (SDS PAGE) electrophoresis, western blotting and chemiluminescent methods. Finally, gel documents software gene tools were used to analyze sub-cellular expression of the transporter.Results: The expression of GLUT4 was considerably higher in the GINGER-treated cells (112.2±2.41) compared to the control (the DMSO-treated cells) (98.62±3.92) (P value<0.05). Also, the amount of GLUT4 in membrane fraction of cells treated with GINGER extract (100±0) was higher compared to the DMSO-treated cells (78.46±5.84). The amount of GLUT4 in cytosolic fraction of cells treated with GINGER extract (12.22±2.41) was lower compared to the control (the DMSO-treated cells) (20.15±2.56). These results show an enhanced translocation of GLUT4 from cytosolic fraction to the cell membrane fraction in the GINGER-treatments.Conclusion: One of the mechanisms and also the most important anti-diabetic effects of GINGER would be to decrease insulin resistance increasing GLUT4 translocation to the cell membrane, which declines following diabetic complications.

Cites

No record.

References

No record.

Cite

APA: Copy

Tajik Kord, Marjan, POORRAJAB, FATEMEH, MOHITI ARDEKANI, JAVAD, Azari, Manizheh, & Raeissi, Alireza. (2016). GINGER ACCELERATES GLUT4 TRANSLOCATION TO THE CELL MEMBRANE OF C2C12 MYOTUBES. MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES, 38(3 ), 34-41. SID. https://sid.ir/paper/47381/en

Vancouver: Copy

Tajik Kord Marjan, POORRAJAB FATEMEH, MOHITI ARDEKANI JAVAD, Azari Manizheh, Raeissi Alireza. GINGER ACCELERATES GLUT4 TRANSLOCATION TO THE CELL MEMBRANE OF C2C12 MYOTUBES. MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2016;38(3 ):34-41. Available from: https://sid.ir/paper/47381/en

IEEE: Copy

Marjan Tajik Kord, FATEMEH POORRAJAB, JAVAD MOHITI ARDEKANI, Manizheh Azari, and Alireza Raeissi, “GINGER ACCELERATES GLUT4 TRANSLOCATION TO THE CELL MEMBRANE OF C2C12 MYOTUBES,” MEDICAL JOURNAL OF TABRIZ UNIVERSITY OF MEDICAL SCIENCES, vol. 38, no. 3 , pp. 34–41, 2016, [Online]. Available: https://sid.ir/paper/47381/en

Related Journal Papers