NEW ASPECT OF SKELETON DIFFERENTIAL STAINING BY ALZARIN RED S AND ALCIAN BLUE

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JALALI M.; FAZEL A.R.

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Paper Information

Journal: PHARMACEUTICAL SCIENCES Year:2004 | Volume:- | Issue:1 Page(s): 11-20

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Information Journal Paper

Title

NEW ASPECT OF SKELETON DIFFERENTIAL STAINING BY ALZARIN RED S AND ALCIAN BLUE

Author(s)

JALALI M. | FAZEL A.R. | Issue Writer Certificate

Keywords

ALIZARIN REDSQ3

ALCIAN BLUEQ3

BONE AND CARTILAGEQ3

Abstract

Using specific skeletons staining with alizarine red S (AR) and ALCIAN BLUE (AB) which is a significant method for of ossification centers during embryogenesis. One major problem in this method was been percipitation of chemical stain which has been resulted in weak to no reaction in different stages of BONE AND CARTILAGE development. The purpose of this study was to develop new way to over come this technical problem. By using high concentration of AR (2.5 volume) combined with less concentration AB (0.5 volume) as well as decreasing processor time from 3 days to 1.5 and also we decreased the acidity of staining solution up to pH 5, we were able to show that after clearing of embryos, the soft tissues are absolutely clear and cartilages and bones were stained perfectly. It seems that with modify processors, dye can penetrate the embryonic tissues and remain in place after penetration. As high acidity may decreased the amount of bone, it is recommended to reduced the rate of acidity.

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APA: Copy

JALALI, M., & FAZEL, A.R.. (2004). NEW ASPECT OF SKELETON DIFFERENTIAL STAINING BY ALZARIN RED S AND ALCIAN BLUE. PHARMACEUTICAL SCIENCES, -(1), 11-20. SID. https://sid.ir/paper/48571/en

Vancouver: Copy

JALALI M., FAZEL A.R.. NEW ASPECT OF SKELETON DIFFERENTIAL STAINING BY ALZARIN RED S AND ALCIAN BLUE. PHARMACEUTICAL SCIENCES[Internet]. 2004;-(1):11-20. Available from: https://sid.ir/paper/48571/en

IEEE: Copy

M. JALALI, and A.R. FAZEL, “NEW ASPECT OF SKELETON DIFFERENTIAL STAINING BY ALZARIN RED S AND ALCIAN BLUE,” PHARMACEUTICAL SCIENCES, vol. -, no. 1, pp. 11–20, 2004, [Online]. Available: https://sid.ir/paper/48571/en

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