PREPARATION OF FUSOGENIC LIPOSOMES ENCAPSULATED WITH CYCLOSPORINE A AND EVALUATION OF ITS IMMUNOSUPPRESSIVE EFFECTS ON HUMAN T-CELLS

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MALAEKEH NIKOUEI B.; JAFARI M.R.; SAJADI S.A.; SAMEE A.

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Journal: PHARMACEUTICAL SCIENCES Year:2005 | Volume:- | Issue:2 Page(s): 1-9

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Title

PREPARATION OF FUSOGENIC LIPOSOMES ENCAPSULATED WITH CYCLOSPORINE A AND EVALUATION OF ITS IMMUNOSUPPRESSIVE EFFECTS ON HUMAN T-CELLS

Author(s)

MALAEKEH NIKOUEI B. | JAFARI M.R. | SAJADI S.A. | SAMEE A. | Issue Writer Certificate

Keywords

CYCLOSPORINE AQ2

FUSOGENIC LIPOSOMESQ2

IMMUNOSUPPRESSIVE EFFECTSQ2

CELLQ2

CULTUREQ2

Abstract

The aim of this study was to prepare fusogenic liposome as a drug delivery carrier for CyA METHODS: In this study liposomes containing CyA were prepared by using dipalmitoylphosphatidylcholine (DPPC) and cholesterol via solvent evaporation method and for FUSOGENIC LIPOSOMES dioleoylphosphatidylethanolamine (DOPE) was added to the formulation. For sizing of liposomes, polycarbonate filters with exact size of 1000, 400 and 100 nm was used. For liposome characterization, liposome size was determined by using particle size analyzer and encapsulation percent was evaluated during two months. Finally in vitro IMMUNOSUPPRESSIVE EFFECTS of liposome's and aqueous solution of CyA were compared on human T-cells by MTT [3- (4, 5- dimethylthiazol- 2-yl)-2, 5-diphenyl tetrazolium bromide] test RESULTS: Light microscopic evaluation of the liposome formulations showed that the liposomes are multi lamellar vesicle (MLV). The sizes of non-fusogenic and FUSOGENIC LIPOSOMES were 1.76±0.006 and 2.122±0.004 µm, and their percent of encapsulation were 79.7±3.00 and 85.67±9.46 %, respectively. In vitro immunosuppressive evaluation by T-cell CULTURE was showed that FUSOGENIC LIPOSOMES have more inhibitory effects on T-cell proliferation than non-fusogenic liposome and aqueous solution of CyA. IC50 of MLV, 1000,400 and 100 nm of FUSOGENIC LIPOSOMES were 6.151x10-6, 4.123x10-5 1.461x10-4 and 1.276x10-4mM, respectively. CONCLUSION: In this study high encapsulation percent and fairly stable liposomes encapsulated with CyA were prepared and the results showed FUSOGENIC LIPOSOMES could be a suitable delivery system for CyA.

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APA: Copy

MALAEKEH NIKOUEI, B., JAFARI, M.R., SAJADI, S.A., & SAMEE, A.. (2005). PREPARATION OF FUSOGENIC LIPOSOMES ENCAPSULATED WITH CYCLOSPORINE A AND EVALUATION OF ITS IMMUNOSUPPRESSIVE EFFECTS ON HUMAN T-CELLS. PHARMACEUTICAL SCIENCES, -(2), 1-9. SID. https://sid.ir/paper/48710/en

Vancouver: Copy

MALAEKEH NIKOUEI B., JAFARI M.R., SAJADI S.A., SAMEE A.. PREPARATION OF FUSOGENIC LIPOSOMES ENCAPSULATED WITH CYCLOSPORINE A AND EVALUATION OF ITS IMMUNOSUPPRESSIVE EFFECTS ON HUMAN T-CELLS. PHARMACEUTICAL SCIENCES[Internet]. 2005;-(2):1-9. Available from: https://sid.ir/paper/48710/en

IEEE: Copy

B. MALAEKEH NIKOUEI, M.R. JAFARI, S.A. SAJADI, and A. SAMEE, “PREPARATION OF FUSOGENIC LIPOSOMES ENCAPSULATED WITH CYCLOSPORINE A AND EVALUATION OF ITS IMMUNOSUPPRESSIVE EFFECTS ON HUMAN T-CELLS,” PHARMACEUTICAL SCIENCES, vol. -, no. 2, pp. 1–9, 2005, [Online]. Available: https://sid.ir/paper/48710/en

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