THE FREQUENCY OF HIGH RISK SUB-TYPES OF HUMAN PAPILLOMAVIRUS IN THE SAMPLES WITH VARYING DEGREES OF CERVICAL NEOPLASIA

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HEYDARPOUR MITRA; SALEHI RASOOL; KAMALI ZAHRA; YARAGHI HOURI

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Journal: JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S) Year:2011 | Volume:28 | Issue:120 Page(s): 1477-1483

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Title

THE FREQUENCY OF HIGH RISK SUB-TYPES OF HUMAN PAPILLOMAVIRUS IN THE SAMPLES WITH VARYING DEGREES OF CERVICAL NEOPLASIA

Author(s)

HEYDARPOUR MITRA | SALEHI RASOOL | KAMALI ZAHRA | YARAGHI HOURI | Issue Writer Certificate

Keywords

PAPILLOMAVIRUSQ3

HIGH RISK PAPILLOMAVIRUSQ3

POLYMERASE CHAIN REACTIONQ2

SQUAMOUS INTRAEPITHELIAL LESIONSQ3

SQUAMOUS CELL CARCINOMAQ3

Abstract

Background: cervical cancer is the second most common cancer of women and one of the most important factors in theirmortality. The role of human PAPILLOMAVIRUS (Hpv) in cervical carcinogenesis has been confirmed, but the high risk subtypes are different in different areas. The purpose of this study was to determine high-risk sub-types of HIV in Isfahan.Methods: This study was a case-control, retrospective and analytical study. We collected the data from 12 paraffin blocks of normal cervical tissue and 36 paraffin blocks of cervical tissue of women with pre-malignant and malignant lesions (scc). After DNA extraction from paraffin blocks existence of HPV DNA was investigated by POLYMERASE CHAIN REACTION (PCR) with the primer HPV General. HPV positive cases were tested with Real - Time PCR to determine the virus subtype.Finding: The prevalence of HPV in patient samples was higher than control cases.50 percent of patients had HPV type 16. The frequency of HPV type 59 HPV types in SCC cases was significantly greater than others. There was not a significant correlation between other sub-types and cervical tissue lesion.Conclusion: This study showed that HPV was present in all samples with cervical lesions. Although the high frequency of HPV type 16 in cervical samples, due to low sample size of this study further studies with larger sample size is needed.

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APA: Copy

HEYDARPOUR, MITRA, SALEHI, RASOOL, KAMALI, ZAHRA, & YARAGHI, HOURI. (2011). THE FREQUENCY OF HIGH RISK SUB-TYPES OF HUMAN PAPILLOMAVIRUS IN THE SAMPLES WITH VARYING DEGREES OF CERVICAL NEOPLASIA. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), 28(120), 1477-1483. SID. https://sid.ir/paper/49764/en

Vancouver: Copy

HEYDARPOUR MITRA, SALEHI RASOOL, KAMALI ZAHRA, YARAGHI HOURI. THE FREQUENCY OF HIGH RISK SUB-TYPES OF HUMAN PAPILLOMAVIRUS IN THE SAMPLES WITH VARYING DEGREES OF CERVICAL NEOPLASIA. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)[Internet]. 2011;28(120):1477-1483. Available from: https://sid.ir/paper/49764/en

IEEE: Copy

MITRA HEYDARPOUR, RASOOL SALEHI, ZAHRA KAMALI, and HOURI YARAGHI, “THE FREQUENCY OF HIGH RISK SUB-TYPES OF HUMAN PAPILLOMAVIRUS IN THE SAMPLES WITH VARYING DEGREES OF CERVICAL NEOPLASIA,” JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), vol. 28, no. 120, pp. 1477–1483, 2011, [Online]. Available: https://sid.ir/paper/49764/en

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