EFFECTS OF KEEPING SEMEN SAMPLES IN 4-6OC ON SPERM MOTILITY, VIABILITY AND DNA DENATURATION

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

NATEGHIAN ZOHREH; DASHTI GHOLAM REZA; BAGHAZADEH SHEKOFEH; GOLSHAN IRANPOUR FARHAD

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S) Year:2016 | Volume:34 | Issue:401 Page(s): 1181-1186

Download Full-Text

Persian Verion

View:

1,218

Download:

Cites:

Information Journal Paper

Title

EFFECTS OF KEEPING SEMEN SAMPLES IN 4-6OC ON SPERM MOTILITY, VIABILITY AND DNA DENATURATION

Author(s)

NATEGHIAN ZOHREH | DASHTI GHOLAM REZA | BAGHAZADEH SHEKOFEH | GOLSHAN IRANPOUR FARHAD | Issue Writer Certificate

Keywords

DNA DENATURATIONQ1

SPERM PRESERVATIONQ1

SPERMATOZOAQ2

Abstract

Background: Mammalian sperm DNA is the most compacted eukaryotic DNA and this compacted DNA can resist against different environmental and chemical factors. The aim of this study was to assess the effects of keeping washed semen sample in refrigerator (4-6 oC) on sperm viability, total motility and DNA integrity in normozoospermic men.Methods: In this experimental study, 13 semen samples of normozoospermic men were washed twice in modified Ham's F10 medium. These samples were kept in refrigerator (4-6 oC) up to 12 days. On the 0 (immediately after sampling), 1st, 2nd, 5th, 7th and 12th days, the proportion of viable, motile, double stranded DNA SPERMATOZOA were examined. Viability and DNA integrity of sperm cells were examined consecutively using eosin-nigrosin and acridine orange staining.Findings: The viability and total motility of sperm cells significantly decreased (P<0.05) during 12 days of storage in refrigerator (4-6 oC). In contrast with sperm viability and motility, DNA integrity was without significant changes up to 12 days of storage.Conclusion: This study suggests that sperm DNA integrity is preserved even after 12 days of keeping semen sample in refrigerator.

Cites

No record.

References

THE EPIDIDYMAL SPERM VIABILITY, MOTILITY AND DNA INTEGRITY IN DEAD MICE MAINTAINED AT 4-6OC

Cite

APA: Copy

NATEGHIAN, ZOHREH, DASHTI, GHOLAM REZA, BAGHAZADEH, SHEKOFEH, & GOLSHAN IRANPOUR, FARHAD. (2016). EFFECTS OF KEEPING SEMEN SAMPLES IN 4-6^OC ON SPERM MOTILITY, VIABILITY AND DNA DENATURATION. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), 34(401), 1181-1186. SID. https://sid.ir/paper/49778/en

Vancouver: Copy

NATEGHIAN ZOHREH, DASHTI GHOLAM REZA, BAGHAZADEH SHEKOFEH, GOLSHAN IRANPOUR FARHAD. EFFECTS OF KEEPING SEMEN SAMPLES IN 4-6^OC ON SPERM MOTILITY, VIABILITY AND DNA DENATURATION. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)[Internet]. 2016;34(401):1181-1186. Available from: https://sid.ir/paper/49778/en

IEEE: Copy

ZOHREH NATEGHIAN, GHOLAM REZA DASHTI, SHEKOFEH BAGHAZADEH, and FARHAD GOLSHAN IRANPOUR, “EFFECTS OF KEEPING SEMEN SAMPLES IN 4-6^OC ON SPERM MOTILITY, VIABILITY AND DNA DENATURATION,” JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), vol. 34, no. 401, pp. 1181–1186, 2016, [Online]. Available: https://sid.ir/paper/49778/en

Related Journal Papers