DIFFERENCE IN QUANTITIES OF BIFIDOBACTERIA FROM THE INTESTINAL MICROFLORA OF INDIVIDUALS WITH TYPE 2 DIABETES AND HEALTHY INDIVIDUALS FROM IRAN

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

NAZEMI MARZIEH; AMINI MASOUD; SALEHI RASOUL

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Isfahan Medical School Year:2013 | Volume:31 | Issue:247 Page(s): 1216-1225

Download Full-Text

Persian Verion

View:

1,050

Download:

Cites:

Information Journal Paper

Title

DIFFERENCE IN QUANTITIES OF BIFIDOBACTERIA FROM THE INTESTINAL MICROFLORA OF INDIVIDUALS WITH TYPE 2 DIABETES AND HEALTHY INDIVIDUALS FROM IRAN

Author(s)

NAZEMI MARZIEH | AMINI MASOUD | SALEHI RASOUL | Issue Writer Certificate

Keywords

TYPE 2 DIABETESQ2

GUT MICROFLORAQ2

BIFIDOBACTERIUMQ2

REAL-TIME PCRQ1

OBESITYQ1

Abstract

Background: OBESITY and Diabetes are two metabolic diseases that are dependent on genetic and various environmental factors. Today, OBESITY and TYPE 2 DIABETES cannot just be attributed to genome changes, bad eating habits, or physical activity reduction. GUT MICROFLORA is another effective factor that has an important role in health. Recently, numerous experiments have been done to investigate the relation between metabolic diseases and bacterial populations in the gut. The purpose of this study is to assess the bifidobacteria of the intestinal microbiota in people with TYPE 2 DIABETES and compare with healthy persons.Methods: In this study, 40 male and female adults, 20 of whom were diagnosed with TYPE 2 DIABETES, were studied. For this study, age, gender, and body-mass index (BMI) are matched in diabetic and healthy persons. After fecal sample collection from diabetic and healthy persons, and extraction of DNA from samples, culture and extraction of DNA from standard bifidobacteria were done as a positive control. After that, cloning in PTZ57 R/T plasmid and extraction cloning plasmid was done.For quantitative investigation of bifidobacteria, REAL-TIME PCR molecular technique was done by using special primer of BIFIDOBACTERIUM and standard curve. To study the number of bifidobacteria in people with TYPE 2 DIABETES and compare with healthy persons, statistical analysis was done.Findings: Average number of bacteria in people with TYPE 2 DIABETES is more than in healthy persons, but Student’s independent t-test (P=0.09) and Mann-Whitney test (P=0.1) indicate that there are no meaningful differences between them.Conclusion: Quantitative calculation with REAL-TIME PCR indicates that the number of bifidobacteria is not effective in Iranian TYPE 2 DIABETES patients.

Cites

No record.

References

No record.

Cite

APA: Copy

NAZEMI, MARZIEH, AMINI, MASOUD, & SALEHI, RASOUL. (2013). DIFFERENCE IN QUANTITIES OF BIFIDOBACTERIA FROM THE INTESTINAL MICROFLORA OF INDIVIDUALS WITH TYPE 2 DIABETES AND HEALTHY INDIVIDUALS FROM IRAN. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), 31(247), 1216-1225. SID. https://sid.ir/paper/49846/en

Vancouver: Copy

NAZEMI MARZIEH, AMINI MASOUD, SALEHI RASOUL. DIFFERENCE IN QUANTITIES OF BIFIDOBACTERIA FROM THE INTESTINAL MICROFLORA OF INDIVIDUALS WITH TYPE 2 DIABETES AND HEALTHY INDIVIDUALS FROM IRAN. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)[Internet]. 2013;31(247):1216-1225. Available from: https://sid.ir/paper/49846/en

IEEE: Copy

MARZIEH NAZEMI, MASOUD AMINI, and RASOUL SALEHI, “DIFFERENCE IN QUANTITIES OF BIFIDOBACTERIA FROM THE INTESTINAL MICROFLORA OF INDIVIDUALS WITH TYPE 2 DIABETES AND HEALTHY INDIVIDUALS FROM IRAN,” JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), vol. 31, no. 247, pp. 1216–1225, 2013, [Online]. Available: https://sid.ir/paper/49846/en

Related Journal Papers