INVESTIGATING THE CORRELATION BETWEEN UBIQUITINATION WITH MOTILITY, MORPHOLOGY, AND DNA METHYLATION IN RAT SPERM

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

RAZAVI SHAHNAZ; KHADIVI FARNAZ; HASHEMI FATEMEH

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Isfahan Medical School Year:2017 | Volume:35 | Issue:445 Page(s): 1151-1155

Download Full-Text

Persian Verion

View:

633

Download:

Cites:

Information Journal Paper

Title

INVESTIGATING THE CORRELATION BETWEEN UBIQUITINATION WITH MOTILITY, MORPHOLOGY, AND DNA METHYLATION IN RAT SPERM

Author(s)

RAZAVI SHAHNAZ | KHADIVI FARNAZ | HASHEMI FATEMEH | Issue Writer Certificate

Keywords

SPERM MOTILITYQ2

DNA METHYLATIONQ1

INFERTILITYQ2

UBIQUITINATIONQ2

Abstract

Background: There are various techniques for treatment of male INFERTILITY, nowadays. At the beginning, evaluations are performed to determine the cause of INFERTILITY and the treatment. These include advanced molecular evaluations and assessment of sperm parameters. There is no study investigating the relationship between sperm parameters as an elementary index of male INFERTILITY, and DNA METHYLATION as an important epigenetic mechanism with rat sperm UBIQUITINATION, so far. The aim of this study was to evaluate the motility, morphology, DNA METHYLATION, and UBIQUITINATION in rat sperm and to determine the relationship between them.Methods: First, 10 male mature rats were kept in experimental condition for 9 weeks (one cycle of spermatogenesis). After sacrificing, their semen samples were used to determine the sperm parameters and smear preparation. Through prepared smear and immunofluorescence assay, percentage of UBIQUITINATIONed and methylated sperm were determined and finally, the correlation coefficient between them was calculated.Findings: There was no significant correlation between the UBIQUITINATION and DNA METHYLATION. However, there was an inverse and significant correlation between the percentage of UBIQUITINATION and morphological abnormalities in spermatozoa (P<0.05). The percentage of ubiquitinized sperm and SPERM MOTILITY showed no significant correlation.Conclusion: UBIQUITINATION, as one of the important molecular processes, prevents the participation of defective sperm in fertilization, and transmission of disorders to next generation. DNA METHYLATION and UBIQUITINATION affect sperm chromatin, but these two processes act in an independent manner.

Cites

No record.

References

No record.

Cite

APA: Copy

RAZAVI, SHAHNAZ, KHADIVI, FARNAZ, & HASHEMI, FATEMEH. (2017). INVESTIGATING THE CORRELATION BETWEEN UBIQUITINATION WITH MOTILITY, MORPHOLOGY, AND DNA METHYLATION IN RAT SPERM. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), 35(445 ), 1151-1155. SID. https://sid.ir/paper/49852/en

Vancouver: Copy

RAZAVI SHAHNAZ, KHADIVI FARNAZ, HASHEMI FATEMEH. INVESTIGATING THE CORRELATION BETWEEN UBIQUITINATION WITH MOTILITY, MORPHOLOGY, AND DNA METHYLATION IN RAT SPERM. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)[Internet]. 2017;35(445 ):1151-1155. Available from: https://sid.ir/paper/49852/en

IEEE: Copy

SHAHNAZ RAZAVI, FARNAZ KHADIVI, and FATEMEH HASHEMI, “INVESTIGATING THE CORRELATION BETWEEN UBIQUITINATION WITH MOTILITY, MORPHOLOGY, AND DNA METHYLATION IN RAT SPERM,” JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), vol. 35, no. 445 , pp. 1151–1155, 2017, [Online]. Available: https://sid.ir/paper/49852/en

Related Journal Papers