THE COMPARISON OF CHARACTERISTICS OF HUMAN OSTEOBLAST CELLS IN TWO METHODS, MONOLAYER CULTURE AND HYDROXYAPATITE-TRICALCIUM PHOSPHATE SCAFOLD

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HASHEMIBENI BATOOL; ALI AKBARI FERESHTEH; SADEGHI FARZANEH; ESFANDIARY EBRAHIM; BAHRAMIAN HAMID; SADEGHI HOSEIN

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Journal: JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S) Year:2011 | Volume:28 | Issue:119 Page(s): 1348-1358

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Title

THE COMPARISON OF CHARACTERISTICS OF HUMAN OSTEOBLAST CELLS IN TWO METHODS, MONOLAYER CULTURE AND HYDROXYAPATITE-TRICALCIUM PHOSPHATE SCAFOLD

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Keywords

MONOLAYER CULTUREQ2

BONE REGENERATIONQ3

HYDROXYAPATITE-CALCIUM PHOSPHATEQ3

OSTEOBLASTQ2

Abstract

Background: Bone diseases and injuries, including bone fractures during accidents and trauma, in all communities are prevalent and in most cases, crushed bone is not able to repair; various treatment ways including tissue graft from an allograft or autograft have risks and lead to problems. One method for treatment of bone fractures is using outologous OSTEOBLASTs with culturing on suitable scaffolds and then implant of cells to defect region. In this study, we cultured OSTEOBLAST cells on monolayer and calcium phosphate hydroxyapatite (HA-TC) scaffolds and the behavior of these two types of cells in different cultures were analyzed.Methods: During the craniotomy operation in Alzahra hospital in five individual, bone samples were obtained and in sterile condition were sent to the laboratory. Each sample after washing with PBS was cut into small pieces and plates containing the medium culture were transferred to the incubator. After 10-12 days, the cells have started to leave the bones covering the floor plate and after using the enzyme Trypsin-EDTA isolated from plate divided into two groups. One group was cultured in HA-TC scaffold and other as MONOLAYER CULTURE. Van kossa staining, flow cytometry, alkaline phosphatase assay and MTT method were used to investigate the characteristics of OSTEOBLASTs.Finding: OSTEOBLASTs in MONOLAYER CULTURE were observed as fibroblast- like cells. Van kossa staining determined the mineral deposits in the two groups. The cell proliferation increased in cellular scaffolding HA-TC more than monolayer (P<0.001). Results of MTT showed more activity items on living cells in scaffold HA-TC than monolayer (P<0.001). In alkaline phosphatase staining, blue granules were observed; in the flow cytometry, 57 percent of primary cultured cells and 5 percent in the third passage cells showed alkaline phosphatase marker.Conclusion: According to the results of this study, the hydroxyapatite scaffold is better than MONOLAYER CULTURE for OSTEOBLAST cells. This could be because of having more calcium and similar elements to bone matrix that facilitate feeding cultured cells.

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APA: Copy

HASHEMIBENI, BATOOL, ALI AKBARI, FERESHTEH, SADEGHI, FARZANEH, ESFANDIARY, EBRAHIM, BAHRAMIAN, HAMID, & SADEGHI, HOSEIN. (2011). THE COMPARISON OF CHARACTERISTICS OF HUMAN OSTEOBLAST CELLS IN TWO METHODS, MONOLAYER CULTURE AND HYDROXYAPATITE-TRICALCIUM PHOSPHATE SCAFOLD. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), 28(119), 1348-1358. SID. https://sid.ir/paper/50382/en

Vancouver: Copy

HASHEMIBENI BATOOL, ALI AKBARI FERESHTEH, SADEGHI FARZANEH, ESFANDIARY EBRAHIM, BAHRAMIAN HAMID, SADEGHI HOSEIN. THE COMPARISON OF CHARACTERISTICS OF HUMAN OSTEOBLAST CELLS IN TWO METHODS, MONOLAYER CULTURE AND HYDROXYAPATITE-TRICALCIUM PHOSPHATE SCAFOLD. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)[Internet]. 2011;28(119):1348-1358. Available from: https://sid.ir/paper/50382/en

IEEE: Copy

BATOOL HASHEMIBENI, FERESHTEH ALI AKBARI, FARZANEH SADEGHI, EBRAHIM ESFANDIARY, HAMID BAHRAMIAN, and HOSEIN SADEGHI, “THE COMPARISON OF CHARACTERISTICS OF HUMAN OSTEOBLAST CELLS IN TWO METHODS, MONOLAYER CULTURE AND HYDROXYAPATITE-TRICALCIUM PHOSPHATE SCAFOLD,” JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), vol. 28, no. 119, pp. 1348–1358, 2011, [Online]. Available: https://sid.ir/paper/50382/en

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