PREVALENCE OF ROTAVIRUS GASTROENTERITIS AND CIRCULATING GENOTYPES IN CHILDREN WITH ACUTE GASTROENTERITIS ADMITTED IN IMAM HOSSEIN HOSPITAL, ISFAHAN, IRAN; 2013

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MOGHIM SHARAREH; FAZELI HOSSEIN; NASR ESFAHANI BAHRAM; JAFARI MODREK HODA

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Journal Paper

Paper Information

Journal: JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S) Year:2013 | Volume:31 | Issue:250 Page(s): 1360-1368

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Title

PREVALENCE OF ROTAVIRUS GASTROENTERITIS AND CIRCULATING GENOTYPES IN CHILDREN WITH ACUTE GASTROENTERITIS ADMITTED IN IMAM HOSSEIN HOSPITAL, ISFAHAN, IRAN; 2013

Author(s)

MOGHIM SHARAREH | FAZELI HOSSEIN | NASR ESFAHANI BAHRAM | JAFARI MODREK HODA | Issue Writer Certificate

Keywords

ROTAVIRUSQ2

GASTROENTERITISQ2

GENOTYPE GQ2

GENOTYPE PQ3

ISFAHANQ3

IRANQ3

Abstract

Background: Human ROTAVIRUS is a significant cause of severe GASTROENTERITIS in infants and young children worldwide. In recent years, ROTAVIRUS genotyping by reverse transcription polymerase chain reaction (RT-PCR) has provided valuable information about the diversity of ROTAVIRUSes circulating worldwide. The purpose of the present study was to monitor the prevalence of the different circulating G and P genotypes of ROTAVIRUSes in ISFAHAN, IRAN.Methods: During the period of spring 2013, a total of 117 stool samples were collected from children less than 3 years old who were hospitalized for acute GASTROENTERITIS in Imam Hossein Hospital, ISFAHAN, IRAN, as the referral pediatrics hospital. ROTAVIRUS-associated diarrhea was investigated in fecal specimens with sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). ROTAVIRUS-positive specimens were typed by the nested reverse transcription (RT) polymerase chain reaction (Nested RT-PCR) using different types of specific primers.Findings: Out of the 117 collected samples, 35.3% (41 cases) tested positive for ROTAVIRUS. The frequency of G1P8, G8P4, G8P8, G1P4 and G1G4P8 types was 65.9%, 17.1%, 12.2%, 2.4% and 2.4%, respectively.Conclusion: In this study, the prevalence ROTAVIRUS infection among children less than 3 years of age in ISFAHAN was determined. The dominant genotyped was G1P8. Other studies in important areas of the country are needed to decide about future vaccine formulation in IRAN.

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APA: Copy

MOGHIM, SHARAREH, FAZELI, HOSSEIN, NASR ESFAHANI, BAHRAM, & JAFARI MODREK, HODA. (2013). PREVALENCE OF ROTAVIRUS GASTROENTERITIS AND CIRCULATING GENOTYPES IN CHILDREN WITH ACUTE GASTROENTERITIS ADMITTED IN IMAM HOSSEIN HOSPITAL, ISFAHAN, IRAN; 2013. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), 31(250), 1360-1368. SID. https://sid.ir/paper/50643/en

Vancouver: Copy

MOGHIM SHARAREH, FAZELI HOSSEIN, NASR ESFAHANI BAHRAM, JAFARI MODREK HODA. PREVALENCE OF ROTAVIRUS GASTROENTERITIS AND CIRCULATING GENOTYPES IN CHILDREN WITH ACUTE GASTROENTERITIS ADMITTED IN IMAM HOSSEIN HOSPITAL, ISFAHAN, IRAN; 2013. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)[Internet]. 2013;31(250):1360-1368. Available from: https://sid.ir/paper/50643/en

IEEE: Copy

SHARAREH MOGHIM, HOSSEIN FAZELI, BAHRAM NASR ESFAHANI, and HODA JAFARI MODREK, “PREVALENCE OF ROTAVIRUS GASTROENTERITIS AND CIRCULATING GENOTYPES IN CHILDREN WITH ACUTE GASTROENTERITIS ADMITTED IN IMAM HOSSEIN HOSPITAL, ISFAHAN, IRAN; 2013,” JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), vol. 31, no. 250, pp. 1360–1368, 2013, [Online]. Available: https://sid.ir/paper/50643/en

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