THE EFFECTS OF DIFFERENT EXTRACTS OF VIOLA ODORATA ON TRICHOMONAS VAGINALISIN CULTURE MEDIUM

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SALEHI LEILA; ASGHARI GHOLAMREZA; YOUSEFI HOSSEIN ALI; YOUSEFI DARANI HOSSEIN

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Journal: Journal of Isfahan Medical School Year:2014 | Volume:31 | Issue:266 Page(s): 2139-2148

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Title

THE EFFECTS OF DIFFERENT EXTRACTS OF VIOLA ODORATA ON TRICHOMONAS VAGINALISIN CULTURE MEDIUM

Author(s)

SALEHI LEILA | ASGHARI GHOLAMREZA | YOUSEFI HOSSEIN ALI | YOUSEFI DARANI HOSSEIN | Issue Writer Certificate

Keywords

VIOLA ODORATAQ2

FRACTIONSQ3

TRICHOMONAS VAGINALISQ2

Abstract

Background: Tichomoniasis is a very common sexually transmitted disease (STD) caused by TRICHOMONAS VAGINALIS. Metronidazol with vast side effects is now the only drug approved for the treatment of this infection in many countries. The effect ofVIOLA ODORATA on several parasites is shown in previous studies. In this study, in an attempt to find an alternative drug, the effect of different extracts of this plant onTrichomonas vaginalis in culture medium was investigated.Methods: Three different FRACTIONS of leave, flower, and root including diethyl ether, ethyl acetate, and water FRACTIONS were prepared from crude extract. The extracts were dried using vacuum rotary evaporator and then, they were used for in-vitro anti-trichomonas vaginalis experiments.Findings: The crude extract of leave (4 mg/ml), flower (4 mg/ml), and root (2 mg/ml) of VIOLA ODORATAshowed 100% growth inhibition (GI) during 24 hours. Diethyl ether fraction of leave, flower, and root ofVIOLA ODORATA in concentration of 6 mg/ml showed 100% growth inhibition during 24 hours. The ethyl acetate fraction of leave, flower, and root revealed 100% growth inhibition in the first 24 hours with the minimum concentration of 20 mg/ml, 25 mg/ml, and 25 mg/ml, respectively. Water fraction of leave, flower, and root showed 100% growth inhibition during 24 hours in concentrations of 5, 3, and 1.5 mg/ml, respectively.Conclusion: Considering the appropriate effect of VIOLA ODORATA on TRICHOMONAS VAGINALIS, more investigation is recommended to convert this plant to an anti-Trichomonas vaginalis drug.

Cites

IN-VITRO EFFICACY OF PLANTAGO LANCEOLATA L. EXTRACTS ON TRICHOMONAS VAGINALIS

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APA: Copy

SALEHI, LEILA, ASGHARI, GHOLAMREZA, YOUSEFI, HOSSEIN ALI, & YOUSEFI DARANI, HOSSEIN. (2014). THE EFFECTS OF DIFFERENT EXTRACTS OF VIOLA ODORATA ON TRICHOMONAS VAGINALISIN CULTURE MEDIUM. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), 31(266), 2139-2148. SID. https://sid.ir/paper/50932/en

Vancouver: Copy

SALEHI LEILA, ASGHARI GHOLAMREZA, YOUSEFI HOSSEIN ALI, YOUSEFI DARANI HOSSEIN. THE EFFECTS OF DIFFERENT EXTRACTS OF VIOLA ODORATA ON TRICHOMONAS VAGINALISIN CULTURE MEDIUM. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)[Internet]. 2014;31(266):2139-2148. Available from: https://sid.ir/paper/50932/en

IEEE: Copy

LEILA SALEHI, GHOLAMREZA ASGHARI, HOSSEIN ALI YOUSEFI, and HOSSEIN YOUSEFI DARANI, “THE EFFECTS OF DIFFERENT EXTRACTS OF VIOLA ODORATA ON TRICHOMONAS VAGINALISIN CULTURE MEDIUM,” JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), vol. 31, no. 266, pp. 2139–2148, 2014, [Online]. Available: https://sid.ir/paper/50932/en

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