INHIBITORY EFFECT OF ELECTROPORATION AND SILVER NANOPRTICLES ON THE GROWTH OF LEISHMANIA MAJOR PROMASTIGOTES: INFLUENCE OF PULSE DURATION

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MAYELIFAR KHADIJE; SAZGARNIA AMENEH; YADEGARI DEHKORDI SAJEDEH; ESHGHI HOSSEIN; ATTARAN NEDA; SOUDMAND SALARABADI SAMANEH

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Journal: MEDICAL JOURNAL OF MASHHAD UNIVERSITY OF MEDICAL SCIENCES Year:2013 | Volume:56 | Issue:4 Page(s): 247-254

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Title

INHIBITORY EFFECT OF ELECTROPORATION AND SILVER NANOPRTICLES ON THE GROWTH OF LEISHMANIA MAJOR PROMASTIGOTES: INFLUENCE OF PULSE DURATION

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Keywords

ELECTROPORATIONQ2

ELECTRIC PULSEQ2

LEISHMANIA MAJORQ3

PROMASTIGOTEQ2

SILVER NANO PARTICLEQ1

Abstract

Introduction: Cutaneous Leshmaniasis (CL) is a parasitic disease which is caused by different flagellated protozoan species of leishmania. The objective of this study was to evaluate the effect of ELECTROPORATION on LEISHMANIA MAJOR PROMASTIGOTEs using different pulse durations in the presence of silver nanoparticles (SNP).Materials and Methods: PROMASTIGOTEs of L. major were cultured in RPMI-1640 medium supplemented with 10% FCS at 27oC. The PROMASTIGOTE suspension with a concentration of 40 106 parasite/ml was prepared. It was incubated in the presence of SNP of 6 nm in two various concentrations. By an ECM 830 electroporator device 8 electrical pulses were applied in four duration widths of 50, 100, 150 and 200 microseconds with an electrical field magnitude of 2500 V/cm and a frequency of 1 Hz. Then 24 hrs later the percentage of promatigote survival was measured in each sample by the MTS method in order to determine the IC50 of SNPs and also the effect of certain electrical pulses in the presence and absence of SNPs.Results: It seems that in the presence of SNPs at 24 micromolar concentration, the contribution of electrical pulses with a duration of 150 microseconds and an electrical field magnitude of 2500 V/cm was successful in reducing the proliferation rate of PROMASTIGOTEs (p<0.004).Conclusion: Electrical pulses in the presence of SNPs can lead to a significant decrease in parasite survival rate.

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APA: Copy

MAYELIFAR, KHADIJE, SAZGARNIA, AMENEH, YADEGARI DEHKORDI, SAJEDEH, ESHGHI, HOSSEIN, ATTARAN, NEDA, & SOUDMAND SALARABADI, SAMANEH. (2013). INHIBITORY EFFECT OF ELECTROPORATION AND SILVER NANOPRTICLES ON THE GROWTH OF LEISHMANIA MAJOR PROMASTIGOTES: INFLUENCE OF PULSE DURATION. MEDICAL JOURNAL OF MASHHAD UNIVERSITY OF MEDICAL SCIENCES, 56(4), 247-254. SID. https://sid.ir/paper/52032/en

Vancouver: Copy

MAYELIFAR KHADIJE, SAZGARNIA AMENEH, YADEGARI DEHKORDI SAJEDEH, ESHGHI HOSSEIN, ATTARAN NEDA, SOUDMAND SALARABADI SAMANEH. INHIBITORY EFFECT OF ELECTROPORATION AND SILVER NANOPRTICLES ON THE GROWTH OF LEISHMANIA MAJOR PROMASTIGOTES: INFLUENCE OF PULSE DURATION. MEDICAL JOURNAL OF MASHHAD UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2013;56(4):247-254. Available from: https://sid.ir/paper/52032/en

IEEE: Copy

KHADIJE MAYELIFAR, AMENEH SAZGARNIA, SAJEDEH YADEGARI DEHKORDI, HOSSEIN ESHGHI, NEDA ATTARAN, and SAMANEH SOUDMAND SALARABADI, “INHIBITORY EFFECT OF ELECTROPORATION AND SILVER NANOPRTICLES ON THE GROWTH OF LEISHMANIA MAJOR PROMASTIGOTES: INFLUENCE OF PULSE DURATION,” MEDICAL JOURNAL OF MASHHAD UNIVERSITY OF MEDICAL SCIENCES, vol. 56, no. 4, pp. 247–254, 2013, [Online]. Available: https://sid.ir/paper/52032/en

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