EFFECTS OF PROTEIN KINASE C INHIBITOR ON THE RESPONSES TO THE VASODILATOR AGENTS IN THE ISOLATED AORTA AND MESENTERIC BED FROM HYPERTENSIVE RATS

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FATEHI M.; FATEHI HASSANABAD Z.; IMEN SHAHIDI M.; RAZAVI TOUSI M.T.

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Journal: Iranian Journal of Basic Medical Sciences Year:2004 | Volume:6 | Issue:4 (20) Page(s): 307-314

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Title

EFFECTS OF PROTEIN KINASE C INHIBITOR ON THE RESPONSES TO THE VASODILATOR AGENTS IN THE ISOLATED AORTA AND MESENTERIC BED FROM HYPERTENSIVE RATS

Author(s)

FATEHI M. | FATEHI HASSANABAD Z. | IMEN SHAHIDI M. | RAZAVI TOUSI M.T. | Issue Writer Certificate

Keywords

HYPERTENSIONQ4

PROTEIN KINASE CQ2

RATQ4

VASODILATORSQ4

Abstract

Numerous in vitro studies indicate that endothelium-mediated relaxation is reduced with development of HYPERTENSION. Considering the role of protein kinases in many metabolic processes of phosphate transferring and its importance in the cell communication and function (in the physiological and pathological states) and also the absence of any reports on the effects of these enzymes in the mediating responses to VASODILATORS during HYPERTENSION, this study became of interest. The objectives of this study were to measure the vascular responses to acetylcholine (ACh) and sodium nitroprusside (SNP) in the isolated RAT aorta and mesenteric bed removed either from hypertensive or control RATs and also to investigate the role of PROTEIN KINASE C in these responses. HYPERTENSION was induced in the male Sprague-Dawley RATs (200-250 g) by DOCA-salt injection (20 mg/kg, twice weekly, for 5 weeks, s.c.) and NaCl (1 %) was added to their drinking water. Control RATs received saline injection (0.5 ml/kg, twice weekly, for 5 weeks, s.c.). 5 weeks later, animals were anaesthetized with thiopental (30 mg/kg, Lp.), and arterial blood pressure was directly measured. Mean arterial blood pressure in control and hypertensive RATs were: 98±7.5, 163± 3.5, mmHg, respectively (P < 0.0001). In in vitro studies, rings of descending aorta were cut and mounted for isometric tension recording in an orian chamber containg Krebs solution. After 1 h of stabilization, rings were precontracted with phenylephrine (5 × 10-8 or 10-6M), then concentration response curve to acetylcholine (ACh, 10-6-10-3M) and SNP (10-8-10-4 M) were constructed. There was a significant decrease in response to Ach and also a reduction in the maximal response in rings isolated trom hypertensive RATs. Mesenteric beds were also removed either from control or hypertensive RATs and perfused with Krebs solution. After 1 h of stabilization, tissue were precontracted with noradernalin (10-6M), then concentration-response curve to ACh (10-8-10-4 M) and SNP (10-8-10-4M) were constructed. Responses to ACh but not to SNP were significantly reduced in tissue removed trom hypertensive RATs (eg. in response to 10-6 M of ACh: control:-41.6 ±4.9 hypertensive: 17.2 ±3.6 mmHg, P < 0.05). However, addition of chelerythrine (10µ M), a PROTEIN KINASE C (PKC) inhibitor, to the organ bath significantly restored these impaired responses. These results suggest that PROTEIN KINASE C is involved in the endothelial dysfunction induced by HYPERTENSION.

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APA: Copy

FATEHI, M., FATEHI HASSANABAD, Z., IMEN SHAHIDI, M., & RAZAVI TOUSI, M.T.. (2004). EFFECTS OF PROTEIN KINASE C INHIBITOR ON THE RESPONSES TO THE VASODILATOR AGENTS IN THE ISOLATED AORTA AND MESENTERIC BED FROM HYPERTENSIVE RATS. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 6(4 (20)), 307-314. SID. https://sid.ir/paper/52479/en

Vancouver: Copy

FATEHI M., FATEHI HASSANABAD Z., IMEN SHAHIDI M., RAZAVI TOUSI M.T.. EFFECTS OF PROTEIN KINASE C INHIBITOR ON THE RESPONSES TO THE VASODILATOR AGENTS IN THE ISOLATED AORTA AND MESENTERIC BED FROM HYPERTENSIVE RATS. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2004;6(4 (20)):307-314. Available from: https://sid.ir/paper/52479/en

IEEE: Copy

M. FATEHI, Z. FATEHI HASSANABAD, M. IMEN SHAHIDI, and M.T. RAZAVI TOUSI, “EFFECTS OF PROTEIN KINASE C INHIBITOR ON THE RESPONSES TO THE VASODILATOR AGENTS IN THE ISOLATED AORTA AND MESENTERIC BED FROM HYPERTENSIVE RATS,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 6, no. 4 (20), pp. 307–314, 2004, [Online]. Available: https://sid.ir/paper/52479/en

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