AN ASSESSMENT OF THE EFFECTS OF DIFFERENT DOSE LEVELS OF GAMMA RAYS ON HPRT GENE OF T-CELLS FROM HUMAN PERIPHERAL BLOOD

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BAHREYNI TOUSI S.M.T.; HAMZEHLUEE T.; REZAEE M.

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Journal: Iranian Journal of Basic Medical Sciences Year:2002 | Volume:5 | Issue:3 (15) Page(s): 127-134

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Title

AN ASSESSMENT OF THE EFFECTS OF DIFFERENT DOSE LEVELS OF GAMMA RAYS ON HPRT GENE OF T-CELLS FROM HUMAN PERIPHERAL BLOOD

Author(s)

BAHREYNI TOUSI S.M.T. | HAMZEHLUEE T. | REZAEE M. | Issue Writer Certificate

Keywords

LETQ4

MPCRQ4

PCRQ2

HPRTQ4

T-IYMPHOCYTEQ4

6-THIOGUANINEQ2

DELETIONQ4

Abstract

Ionizing radiation has been shown to produce a broad variety of genetic alterations in humans and other species. Most genetic alterations are deLETions. To study genetic alterations, assessment of somatic cell gene mutations induced by ionizing radiation is the proper method. In this study, intragenic and total gene deLETions were analyzed in gamma ray induced 18HPRT mutants derived from T-lymphocytes PCR amplification of individual HPRT exons and multiplex PCR. HPRT mutants isolated by treatment of irradiated samples with 6-THIOGUANINE.mPCR and PCR of individual exons of HPRT demonstrated that the intragenic and total gene deLETions were not significantly different. In addition, the correlation between relative mutant cell numbers and different doses of gamma rays were determined. In this correlation, relative mutant number increased with great a slope in doses higher than 2 GY. Mapping of all intragenic deLETion break points exhibited a nonrandom distribution of break points. The middle portion of HPRT gene was sensitive to gamma rays. The sensitivity was elevated by radiation dose. This study showed that the size of deLETion appeared to be dependent on dose. Our results suggest that alteration in T-lymphocyte mutant numbers induced deLETions, size of deLETions and distribution of DNA break points appears to be dependent on radiation dose of low LET.

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APA: Copy

BAHREYNI TOUSI, S.M.T., HAMZEHLUEE, T., & REZAEE, M.. (2002). AN ASSESSMENT OF THE EFFECTS OF DIFFERENT DOSE LEVELS OF GAMMA RAYS ON HPRT GENE OF T-CELLS FROM HUMAN PERIPHERAL BLOOD. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 5(3 (15)), 127-134. SID. https://sid.ir/paper/52502/en

Vancouver: Copy

BAHREYNI TOUSI S.M.T., HAMZEHLUEE T., REZAEE M.. AN ASSESSMENT OF THE EFFECTS OF DIFFERENT DOSE LEVELS OF GAMMA RAYS ON HPRT GENE OF T-CELLS FROM HUMAN PERIPHERAL BLOOD. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2002;5(3 (15)):127-134. Available from: https://sid.ir/paper/52502/en

IEEE: Copy

S.M.T. BAHREYNI TOUSI, T. HAMZEHLUEE, and M. REZAEE, “AN ASSESSMENT OF THE EFFECTS OF DIFFERENT DOSE LEVELS OF GAMMA RAYS ON HPRT GENE OF T-CELLS FROM HUMAN PERIPHERAL BLOOD,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 5, no. 3 (15), pp. 127–134, 2002, [Online]. Available: https://sid.ir/paper/52502/en

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